'Recognition site' diaporamas de présentation

Fall 2008

Fall 2008. G e l E l e c t r o p h o r e s i s. Electrophoresis is a molecular technique that separates nucleic acids and proteins based on Size and +-+ Charge +-+. Shape. DNA is a negatively charged molecule and therefore is attracted to positive charges.

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BIOTECHNOLOGY: PRINCIPLES, PROCESSES AND APPLICATIONS

BIOTECHNOLOGY: PRINCIPLES, PROCESSES AND APPLICATIONS. PROJECT DESIGNERS G R KRISHNA CHAND AVATAR ROLL - 8 GINNGAIHLIAN ROLL - 7. CERTIFICATE.

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Encoding Information for DNA computing

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FCH 532 Lecture 16

FCH 532 Lecture 16. Chapter 31. Pili/Fimbriae. Exclusively in Gram - bacteria Short, fine, hair-like appendages (hollow core) Composed of helical protein subunits Functions Not involved in motility.

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Lab Review: 6-12

Lab Review: 6-12. Lab 6: Molecular Biology. Lab 6: Molecular Biology. Description Transformation insert foreign gene in bacteria by using engineered plasmid also insert ampicillin resistant gene on same plasmid as selectable marker Gel electrophoresis cut DNA with restriction enzyme

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DNA

DNA. DNA. Deoxyribonucleic acid Instructions for life Nucleus Double helix Arranged in chromosomes. Gene . * Basic unit of heredity * Segment of a chromosome that codes for a protein. Humans have 46 chromosomes 23 pairs in every cell except for: A. RBCs B. eggs and sperm (1/2)

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Lab 3: Mitosis & Meiosis

Lab 3: Mitosis & Meiosis. Conclusions Mitosis longest phase = interphase each subsequent phase is shorter in duration Meiosis 4:4 arrangement in ascospores no crossover any other arrangement crossover 2:2:2:2 or 2:4:2. total crossover. % crossover. =. total offspring.

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Mark Chaskes and Paul Lazarescu Mentor: Tamar Ratner The Schulich Faculty of Chemistry

Challenging Traditional Approaches to Computation A Biomolecular Transducer Employing Ternary Language and Rendering a Biological Output. Mark Chaskes and Paul Lazarescu Mentor: Tamar Ratner The Schulich Faculty of Chemistry Technion, Haifa, Israel, 32000. Objective.

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Electrophoresis and Blots

Electrophoresis and Blots. Principles recap. Nucleic acids (DNA, RNA) move from the negative to the positive pole during gel electrophoresis The resistance to movement is due to the sieving effect of the gel matrix The rate of migration is related to the size of the nucleic acid.

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What is Gene Technology?

What is Gene Technology?. Gene technology is a broad field which includes analysis of DNA as well as genetic engineering and other forms of genetic modification. Genetic engineering refers the artificial manipulation of genes: adding or subtracting genes, or changing the way genes work.

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Orientation Lab Safety and Restriction Enzymes

Orientation Lab Safety and Restriction Enzymes. Kabi Neupane, Ph.D. Leeward Community College. Objectives. Familiarize with laboratory safety Learn about Restriction enzymes Perform a restriction digestion of Arabidopsis genomic DNA. Laboratory Safety. Emergency procedures

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Restriction Enzymes

Restriction Enzymes. Presented by: Elizabeth Gordon April 11,2006. Function. (From Tock and Dryden 2005). Historical Perspective. (From Lewin 2003). Four Types. (From Tock and Dryden 2005). Type I. Three subunits form R 2 M 2 S 1

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DNA and Restriction Enzymes

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Anatomy of Procaryotic Cells

Anatomy of Procaryotic Cells. Cell Wall. Wall structure based on peptidoglycan polysaccharide composed of 2 sugars N-acetylglucoseamine NAG N-acetylmuramic acid polypeptide short amino acid chains that link the polysaccharides together. Gram + cell wall. Many layers of peptidoglycan

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Restriction Enzymes and DNA Fingerprinting

Restriction Enzymes and DNA Fingerprinting. Molecular Scissors for Cutting DNA Precisely. Its because of these biological catalysts that genetic engineering is possible Restriction enzymes can also be called Endonucleases . Types of Restriction Enzymes. Type I

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Biotechnology

Biotechnology. DNA technology. Review. Some of the most important techniques used in biotechnology involve making recombinant DNA molecules Recombinant objects have been reassembled from parts taken from more than one source Bicycles. Recombinant DNA.

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Lab 2 Restriction Digestion of Plasmid DNA ( pARA and pKAN -R)

Lab 2 Restriction Digestion of Plasmid DNA ( pARA and pKAN -R). Lab 2. Pre Lab Readiness . Familiarity and proper use of micropipettes Remember the 1 st and 2 nd stops Understand enzymes Not consumed in reaction Affected by time, pH, temp Biological catalyst made of protein

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SUPPLEMENTARY DATA

SUPPLEMENTARY DATA. Figure S1. The experimental strategy to determine the linking number difference of plasmids in the presence of a sequence-specific DNA-binding protein and in its absence. (A) The ligation method. (B) The Topo I (topoisomerase I) method.

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DNA FORENSICS

DNA profile from sexual and violent criminals automatically entered into database. DNA samples from crime scenes collected and entered into database. Match!. Offender Index. Forensic Index. Victim / Perpetuator / Accomplice Identified. DNA FORENSICS.

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