Reference genes - PowerPoint PPT Presentation

Homework 7

Homework 7. Planning the experiment. Biological problem. this will be very tricky to plan. Publication. if you do not know what is required here. ?. … … … … … … … … … … …. etc.…. This homework is designed to help you to make this list. which will then guide your experiment plan.

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Sample (dissected frozen brain region)

Data generation. Sample (dissected frozen brain region). RNA extraction (total RNA) QIAZOL and RNeasy mini kit. RNA quantification (Bioanalyser). Reverse transcription MMLV RT-ase Random priming. Q-PCR with gene-specific primers Multiple reference genes Genes of interest.

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