'Related proteins' diaporamas de présentation

Developing and Using Special Purpose Hidden Markov Model Databases

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Ch 3 Sequence Alignment

Ch 3 Sequence Alignment. Pairwise alignments. Introduction Why do alignments? Definitions Dot matrix comparison Scoring alignments Alignment methods Significance of alignments. Why do alignments?.

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Li & Kelly SV40 DNA replication In vitro. PNAS (1984) 81:6973

Li & Kelly SV40 DNA replication In vitro. PNAS (1984) 81:6973. What else could they do to validate their system?. Fractionation to homogeneity. ELONGATION FACTORS ARE CONSERVED. RF-C. RF-C. RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs

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Sequence Alignments and Database Searching 08/20/07

Sequence Alignments and Database Searching 08/20/07. Protein A of interest to you. ornithine decarboxylase?. Why compare protein sequences?. Significant sequence similarities allow associations based upon known functions. Homology vs. similarity.

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Neuronal Cytoskeleton: Structure and Function

Neuronal Cytoskeleton: Structure and Function. Cytoskeleton. Eukaryotic cell Skeletal System Three well defined filamentous structures Microtubules Microfilaments Intermediate filaments. Cytoskeleton. Eukaryotic cell Skeletal System Micotubules Rigid tubes Tubulin Microfilaments

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Sequence Alignment

Sequence Alignment. Outline. Global Alignment Scoring Matrices Local Alignment Alignment with Affine Gap Penalties. From LCS to Alignment: Change up the Scoring.

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August 26, 2009 Biochemistry 201 Thang Chiu, MEB 7E1, x2024

Sequence Alignments and Database Searching. August 26, 2009 Biochemistry 201 Thang Chiu, MEB 7E1, x2024. Adapted from DKW lecture. Protein A of interest to you. ornithine decarboxylase?. Why compare protein sequences?.

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Aug 27, 2008 Biochemistry 111 Thang Chiu, MEB 7E1, x2024

Sequence Alignments and Database Searching. Aug 27, 2008 Biochemistry 111 Thang Chiu, MEB 7E1, x2024. Adapted from DKW lecture. Protein A of interest to you. ornithine decarboxylase?. Why compare protein sequences?.

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August 25, 2010 Biochemistry 201 David Worthylake, 7152 MEB, x5176

Sequence Alignments and Database Searching. August 25, 2010 Biochemistry 201 David Worthylake, 7152 MEB, x5176. Protein A of interest to you. ornithine decarboxylase?. Why compare protein sequences?. Significant sequence similarities allow associations based upon known functions.

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Pectin

Pectin. Wen Dong 10/15/2010. Structure Function Biosynthesis. Pectin. The plant cell wall is composed of polysaccharides and proteins. The wall polysaccharides are often classified into cellulose, hemicelluloses and pectin.

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Protein Sequence

Protein Sequence. Amino Acid Composition IEC RP HPLC Ancient Sequencing methods Modern Sequencing methods Sequencing the Gene Then what?. Amino Acid Composition. 1952 - Complete Acid Hydrolysis Ion Exchange Chromatography with programmed buffer changes (~3 hr)

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Roadmap

Roadmap. The topics: basic concepts of molecular biology more on Perl overview of the field biological databases and database searching sequence alignments phylogenetics structure prediction microarray data analysis. Sequence alignments. Introduction What is an alignment?

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Computational biology

Computational biology. Outline. Proteins DNA RNA Genetics and evolution The S equence M atching Problem RNA Sequence Matching Complexity of the Algorithms. DEFINITION.

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Group Members: Wayne Christopher, Ph.D. Bob Edgar, Ph.D. Duncan Brown Dan Holliman

UC Berkeley Phylogenomics Kimmen Sj ö lander http://phylogenomics.berkeley.edu kimmen@uclink.berkeley.edu Algorithm & tool development for protein structure-function prediction. Investigation of innate immunity in plants and animals: Brian Staskawicz (Plant & Microbial Biology)

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Interferon

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het -genes in Aspergillus niger

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Protein Evolution and Sequence Analysis

Protein Evolution and Sequence Analysis. Central Premise. Significant sequence similarity allows one to assign function to an unknown protein(s) based on properties of known proteins and is a direct consequence of evolutionary relationships.

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a) Normal learning

BAX. BAX. BAD. CASP3. CASP3. pCASP9. pCASP9. BAX. pCASP9. BCL2. BAD. BAD. BCL2. BCL2. CASP3. BAD. BAX. BAX. BCL2. BAX. pCASP9. CASP3. CASP3. pCASP9. pCASP9. BAD. BCL2. BAD. CASP3. BCL2. Supplementary Figure S1. Hippocampus, Apoptosis related proteins.

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Supplementary Fig. 1

Supplementary Fig. 1.

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