'Residue mass' diaporamas de présentation

Proteomics via Mass Spectrometry (a bioinformatics perspective)

Proteomics via Mass Spectrometry (a bioinformatics perspective). Vineet Bafna www.cse.ucsd.edu/~vbafna. Nobel Citation 2002. Nobel Citation, 2002. Proteomics via MS. Enzymatic Digestion (Trypsin) +. Fractionation. Q: Sufficient to identify peptides?. Peptide MS.

1.7k views • 148 slides

Proteomics via Mass Spectrometry (a bioinformatics perspective)

Proteomics via Mass Spectrometry (a bioinformatics perspective). Vineet Bafna www.cse.ucsd.edu/~vbafna. Nobel Citation 2002. Nobel Citation, 2002. Proteomics via MS. Enzymatic Digestion (Trypsin) +. Fractionation. Q: Sufficient to identify peptides?. Peptide MS.

1.53k views • 148 slides

Week 3: Functional Genomics via Mass Spectrometry

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Week 4: Functional Genomics via Mass Spectrometry

1.15k views • 115 slides

876 views • 76 slides

766 views • 76 slides

CSE182-L12

CSE182-L12. Mass Spectrometry Peptide identification. Mass-Charge ratio. The X-axis is not mass, but (M+Z)/Z Z=1 implies that peak is at M+1 Z=2 implies that peak is at (M+2)/2 M=1000, Z=2, peak position is at 501 Quiz: Suppose you see a peak at 501. Is the mass 500, or is it 1000?.

678 views • 43 slides

CSE182-L12

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Lecture 10 Interpretation of Mass Spectra Peptide Mass Fingerprinting MS/MS sequencing

Lecture 10 Interpretation of Mass Spectra Peptide Mass Fingerprinting MS/MS sequencing. Sample preparation. Protein mixture. Sample separation and visualisation Comparative analysis Digestion. Peptides. Mass spectrometry. MS data. Database search. Protein identification.

855 views • 43 slides

Lecture 10 Interpretation of Mass Spectra Peptide Mass Fingerprinting MS/MS sequencing

Lecture 10 Interpretation of Mass Spectra Peptide Mass Fingerprinting MS/MS sequencing. Sample preparation. Protein mixture. Sample separation and visualisation Comparative analysis Digestion. Peptides. Mass spectrometry. MS data. Database search. Protein identification.

470 views • 43 slides

Interpretation of Mass Spectra

442 views • 42 slides

CSE182-L12

534 views • 40 slides

CSE182-L12

474 views • 40 slides

CSE182-L12

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CSE182-L12

418 views • 34 slides

CSE182-L8

CSE182-L8. Mass Spectrometry. Bio. quiz. What is a gene? What is a transcript? What is translation? What are microarrays? What is a b-ion? What is a y-ion?. 100. 200. 300. 400. 500. De Novo Interpretation: Example. 0 88 145 274 402 b-ions.

315 views • 31 slides

Protein sequencing and Mass Spectrometry

Protein sequencing and Mass Spectrometry. Enzymatic Digestion (Trypsin) +. Fractionation. Sample Preparation. Single Stage MS. Mass Spectrometry. LC-MS: 1 MS spectrum / second. Tandem MS. Secondary Fragmentation. Ionized parent peptide. The peptide backbone.

455 views • 26 slides