'Selectable marker' diaporamas de présentation

Methods of Gene Transfer

Methods of Gene Transfer. Transgenic versus Cloning. Transgenic : creation of transgenic animal or plant (introduction of foreign gene into organism) transgenic organisms produced by introduction of foreign gene into germ line (transgenic offspring!!!)

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Reason:

Screening technique. Technique which is exploited to screen the transformation product (transformant Cell). Reason:

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C. Elegans

C. Elegans. Purpose of the Lab. To learn about DNA Inject DNA into living organisms in an attempt to have the offspring express the traits Stop gene silencing OVERALL: Develop a mechanism to put genes into the germline of the organism so they are passed down (successful transformation).

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Chapter 20 part 3: A Little More Advanced Biotechnology Tools

Chapter 20 part 3: A Little More Advanced Biotechnology Tools. Better Plasmids . Engineered plasmids. Building custom plasmids restriction enzyme sites antibiotic resistance genes as a selectable marker. Eco RI. Selectable marker antibiotic resistance gene on plasmid

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Zone of Inhibition

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Monday, October 13, 2008 Chapter 9, Part II (of III)

Monday, October 13, 2008 Chapter 9, Part II (of III). Assignment : Chapter 9 problems 1-4, 6, 9, 16, 20, 25, 26, 32 Chapter 7 problems due this week and QUIZ!. Cloning. Molecular Cloning. Step 1: Ligation of DNA into vectors Sticky ends Different types of vectors Step 2: Transformation

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Presentation on genome sequencing

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Tian He 2008. 07. 06

Cultivation and Transformation of Yeasts. Tian He 2008. 07. 06. Media. non-selective: YPD without antibiotics Selective: Synthetic complete dropout medium (SC) (auxotroph) YPD with antibiotics. YPD: 1000 mL Yeast Extract 10 g Peptone (Tryptone) 20 g

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Protein Expression of CHO cells during Dihydrofolate Reductase Mediated Gene Amplification

Protein Expression of CHO cells during Dihydrofolate Reductase Mediated Gene Amplification. 2006 ? 6 ? 12 ? ??? ?? 2 ??? ? ?. dhfr- CHO and the amplifiable selectable marker, DHFR. DHFR. Gene of interest. most popular mammalian expression system within the pharmaceutical

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