A. B. 5 ’- LTR. U6.2. shRNA. CMV. GFP. shRNA CONSTRUCT Scrambled GGATCCC GACGATTCGAGGCGCAGTGAT TTGATATCCG ATCACTGCGCCTCGAATCGT CTTTTTTCCAACTCGAG Cyp2b-KD2 GGATCCC AAGAACACTGAGGTGTACCCC TTGATATCCG GGGGTACACCTCAGTGTTCTT TTTTTTCCAACTCGAGBy nonnie
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Yoon et al. Supplemental Figure S2. B. A. Days. Days. 1. 4. 7. 10. 1. 4. 7. 10. Scr-shRNA. DMSO. AGS Spheroid. AGS Spheroid. 6 μ m. 19 μ m. 41 μ m. 67 μ m. 6 μ m. 18 μ m. 40 μ m. 64 μ m. Smo-shRNA. Vis. 6 μ m. 12 μ m. 18 μ m. 19 μ m. 6 μ m. 12 μ m.
Supplemental Fig. 2. rAAV2-Cont shRNA. a. b. 1. rAAV2-Cont shRNA (Cont shRNA ) 2. rAAV2-mTOR shRNA ( mTOR shRNA ). rAAV2-mTOR shRNA. pH1. pCMV. GFP. pol A. shRNA. Bi-directional promoter. 4 days. 2 days. d. Cont. mTOR. Cont. mTOR. c. mTOR. P- mTOR (S2448). *.
construct. verb. Egyptians constructed pyramids thousands of years ago. I will construct a persuasive essay. He constructed a fort made out of blankets. to build. examine. verb. To solve a crime, detectives might examine footprints. A teacher will examine my test for errors.
Understand the Reasoning. Reflect and Extend. Devise a Plan. Create Prototype. Generate Ideas. Know Why. Understand/Research the Problem. Think Like An Expert. Think Across Disciplinary Boundaries. Handle New Ethical Situations. Construct. Resolve Dilemmas. Carry Out the Plan.
CONSTRUCT. Construct. DEFINITION. Construct (v.) Build or make something. It is used typically when we speak about the road, building or machine. Example The ministry of transport construct a new highway. The municipality is planning to construct a new metro line. SYNONYMS. Build Make
Construct. Chain. There is no word for “of” in Hebrew. #r<a'h' %l,m,. vyaih' lwOq. B The voice of the man. B The king of the land. %l,m,h; db,[,. B The servant of the king. You can have multiple constructs, but only one absolute. %l,m,h; db,[,. B The servant of the king. Construct.
Construct Validity. PUSH. CONSTRUCT VALIDITY Factor analysis: Bivariate correlations. CONSTRUCT VALIDITY Factor analysis (Principal component analysis). Best solution = 8 factors Practice Improvement Learning* Reassurance, Confirmation Recall Frustration not enough info
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Social Construct. Deviance, including sexual deviance, is a social construct. As a social construct, the media is directly responsible for influencing and redefining the meaning of sexual deviance. Male Sports Stars and “Legitimate Deviance”.
f1 origin. A. p NosTerFRT-neo (5500bp). Amp r. Ori. Bgl II Xba I Not I Sac I Sfi I Fse I Avr II …… Not I Xba I Not I Dsa I Sac I. FRT. FRT. Nos-ter. Tn5. pGK. neo. 0.3kb. 1.8kb. UPSTREAM MULTIPLE CLONING SITE