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Research Techniques Made Simple: Small Interfering RNA (siRNA)

Research Techniques Made Simple: Small Interfering RNA (siRNA). Vinod E. Nambudiri, MD MBA 1,2 , Rachael Clark, MD PhD 3 , Hans Widlund , PhD MSc 3 1. Harvard Combined Dermatology Residency Program, Boston, Massachusetts

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Supplementary Table 1 oligo nucleotide sequences for RNA interference

Supplementary Table 1 oligo nucleotide sequences for RNA interference. Underlined sequences were also used for siRNAs for PTB knock down. Supplementary Table 2 competitor oligos and primer sequences for real time PCR.

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Suppl Figure 1. dcl2-1 dcl3-1 dcl4-2. dcl3-1 dcl4-2. dcl2-1 dcl4-2. dcl2-1 dcl3-1. A. dcl3-1. dcl4-2. dcl2-1. Col0. - + - + - + - + - + - + - + - +. 24-nt. CMV (-) siRNAs. 22-nt. 21-nt. miR173. U6. B. dcl2-1 dcl3-1 dcl4-2.

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