Supplementary Figure 1. UPF1 protein. KD - UPF1 UPF1 100% 33% 11% shRNA1 shRNA2. relative mRNA levels. UPF1. Smbb ’. no KD shRNA1 shRNA2. SMG6 protein. KD - SMG6 SMG6 100% 33% 11% shRNA1 shRNA2.By lesley-schneider
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Protein stability, protein-protein interactions. 11-1. Protein stability - how proteins achieve stability under extreme conditions (class presentation) Protein-protein interactions - recent literature (presentations). Stabilization/destabilization forces affecting proteins. 11-2.
Protein protein interactions. Better understanding of protein b’s function. Introduction. Protein b unknown. Protein a known. Interaction. Detecting relationship between pathways. Detecting new pathways. Introduction. Protein a Function a. Protein b Function b. Interaction.
Protein-protein interactions. Chapter 12. Stable complex: homodimeric citrate synthase. Transient Signaling Complex Rap1A – cRaf1. Multi-domain protein. Interface 4890 Å 2. Interface 1310 Å 2. Stable vs . transient protein-protein interactions. Transient Interaction. Stable complex.
Protein-protein interactions. Masoud Youssefi, MD,PhD Division of microbiology/virology. Introduction. important field in cell biology, biochemistry Localization and trafficking posttranslational modifications signaling networks also important field in viral replication
Protein – Protein Interactions. Implementation/Algorithm. Algorithm approximates the minimum set of domains pairs. Algorithm needs to be able to choose d-d pairs in an educated, not a randomized fashion.
Protein-Protein Interactions. Protein Interactions. A Protein may interact with: Other proteins Nucleic Acids Small molecules. Protein-Protein Interactions: The “ Interactome ”. Experimental methods: Mass Spec, yeast 2-hybrid system, microarrays,… Computational techniques:
Protein-protein Interactions. Outline. Introduction The Study of Protein-protein Interaction by Mass Spectrometry Yeast-Two-Hybrid Phage Display Protein-protein Interaction on the Web Information Extraction (IE). Introduction The Study of Protein-protein Interaction by Mass Spectrometry
Protein-Protein Interactions. Genome. Transcriptome. Proteome. Interactome. Atomic NMR spectroscopy X-ray crystallography: co-crystallisation Electron microscopy Molecular modelling: docking Protein Data Bank. Experimental methods to identify PPI’s. Biochemical/biophysical binary
Protein-protein Interactions. Hsueh-Fen Juan 2003, Mar 31 NTNU. Outline. Introduction The Study of Protein-protein Interaction by Mass Spectrometry Yeast-Two-Hybrid Phage Display Protein-protein Interaction on the Web Information Extraction (IE). Introduction
Protein-protein interactions. Three types of interactions. Interactions between domains in multidomain proteins Interactions between the domains in stable complexes Interactions between proteins in transient interactions. Stable complexes.