A. Synthetic siRNA against STAT3. 5’-CAU CUG CCU AGA UCG GCU AdTdT-3’ 3’-dTdT GUA GAC GGA UCU AGC CGA U- 5’. Vehicle Stat3 siRNA GFP siRNA. Vehicle Stat3 siRNA GFP siRNA. Vehicle Stat3 siRNA. B. a -STAT3 a - b -actin. a -STAT3 a -MAPK. A172. U251. NHA. C.By naasir
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Supplemental Figure 2. Contrl siRNA + - PIK3R3 siRNA - +. A. B. PIK3R3. actin. C. Control siRNA PIK3R3 siRNA. D. Control siRNA. PIK3R3 siRNA. Brdu -FITC. Annexin -V. 2.9%. 2.58%. 7-ADD. PI.
p < 0.01. Nonspecific siRNA Noxa siRNA. Supplemental Figure 4. Noxa mediates synergism by the ABT-737/etoposide combination. UM-22A cells were transfected with 100 nM Noxa siRNA (5’-GUAAUUAUUGACACAUUUCTT-3’)
Supplemental data S1. SW480. Control siRNA BRG1 siRNA. PTEN – Akt – p-Akt – p-GSK-3 b – Cyclin D1 – BRG1 – b -actin –.
Selected siRNA. Control siRNA. A. B. CTSS. CTSS. Selected siRNA. Control siRNA. 1 0.2 . CD137L. CD137L. 1 0.1 . EFNA3. MDK. 1 0.2. 1 0.2 . GDF15. NEO1. 1 0.2 . 1 0.1 . GTF2I. SERPINI1. 1 0.5 . 1 0.2 . MDK. PODXL.
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Supplemental Figure 3. A. B. Ctrl siRNA. PIK3R3 siRNA. Ctrl siRNA + - PIK3R3 siRNA - +. p-RB (ser 780). Cyclin D1. p-AKT (ser 473). p- mTOR (ser 2448). PIK3R3. actin.
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