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?? DNA ?? Recombinant DNA Technology

?? DNA ?? Recombinant DNA Technology

?????. ?? DNA ?? Recombinant DNA Technology. ???? ??????? ?? ???? ????????? ?????. ?? (clone) ??????????????????. ?????????????? (cloning) ???????. ????????? (molecular clone) ? DNA ?? (DNA cloning) ???? ????????? ????????????. ?? DNA ?? ? recombinant DNA technology ?

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2.48k views • 207 slides
?? DNA ?? Recombinant DNA Technology

?? DNA ?? Recombinant DNA Technology

?????. ?? DNA ?? Recombinant DNA Technology. ???? ??????? ?? ???? ????????? ?????. ?? (clone) ??????????????????. ?????????????? (cloning) ???????. ????????? (molecular clone) ? DNA ?? (DNA cloning) ???? ????????? ????????????. ?? DNA ?? ? recombinant DNA technology ?

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2.33k views • 207 slides
Chapter 14 Genetic Recombination and Genetic Engineering

Chapter 14 Genetic Recombination and Genetic Engineering

Chapter 14 Genetic Recombination and Genetic Engineering. The biochemistry and molecular biology department of CMU. Section 1 DNA Recombination. DNA recombination. Homologous Recombination Conjugation Transformation Transduction Site-specific Recombination Transposition.

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1.31k views • 79 slides
Chapter 14 Genetic Recombination and Genetic Engineering

Chapter 14 Genetic Recombination and Genetic Engineering

Chapter 14 Genetic Recombination and Genetic Engineering. The biochemistry and molecular biology department of CMU. Section 1 DNA Recombination. DNA recombination. Homologous Recombination Conjugation Transformation Transduction Site-specific Recombination Transposition.

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1.17k views • 79 slides
??????? Polymerase chain reaction ? PCR ?

??????? Polymerase chain reaction ? PCR ?

??????? Polymerase chain reaction ? PCR ?. ??????? ??? shengdq@ctgu.edu.cn. ??? ? ??? ? ?????. PCR ??. PCR ???????????? DNA ?? ????????? ???????????? ?????????. ? ?. Polymerase chain reaction (PCR) ?? K. Mullis ? 1985 ??? , ???????? DNA ??????????????? DNA ???

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1.44k views • 77 slides
??? ???? ?????? ??????

??? ???? ?????? ??????

??? ???? ?????? ??????. The Polymerase Chain Reaction (PCR). By Dr. NAGLAA FATHY Lecturer of Biochemistry & Molecular Biology Faculty of Medicine Benha University E-mail : naglaa_fathy722000@yahoo.com Nagla.alhusseini@fmed.bu.edu.eg. What is the Polymerase Chain Reaction?.

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533 views • 37 slides
Working summary

Working summary

Working summary. Wang Qian. Analyse the 16SrDNA fragment by DGGE. The samples come from China. DNA extraction : use the Wizard Genomic DNA Purification kit (Promega TM , mouse tail protocol) The primers are 16S-SR and 16S-SF-GC. The machine of Dgge is INGENYphorU-2 system.

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418 views • 32 slides
Writtin by: Michael A. Innis, Kenneth B. Mayambo, David H. Gelfand, and Mary Ann D. Brow

Writtin by: Michael A. Innis, Kenneth B. Mayambo, David H. Gelfand, and Mary Ann D. Brow

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Writtin by: Michael A. Innis, Kenneth B. Mayambo, David H. Gelfand, and Mary Ann D. Brow. DNA sequencing. At the time Sagner dideoxynucleotide method used to sequence DNA

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335 views • 18 slides
RAPD Randomly Amplified Polymorphic DNA

RAPD Randomly Amplified Polymorphic DNA

RAPD Randomly Amplified Polymorphic DNA. RAPD. - a method based on PCR developed in 1990. - RAPD is different from conventional PCR as it needs one primer for amplification. The size of primer is normally short (10 nucleotides), and therefore, less specific.

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2.01k views • 16 slides
PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction. PCR. - a method for amplifying (copying) small amount of DNA in nearly any amount required, starting with a small initial quantity. - an in vitro or cell-free method for synthesizing DNA.

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237 views • 14 slides
Cloning Vector Map

Cloning Vector Map

Cloning Vector Map. Vector Characteristics. Topoisomerase. Contains a T-overhang Linked topoisomerase. Topoisomerase. PCR Fragment Properties. A. PCR Product. A. Contains an A-overhang A-T base pairing helps anchor the insert to vector. Topoisomerase. PCR Product. A. A.

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716 views • 8 slides
POLYMERASE CHAIN REACTION

POLYMERASE CHAIN REACTION

POLYMERASE CHAIN REACTION. PCR. Image Credit: Taq DNA polymerase . Features. Amplifies small samples of DNA into millions of copies Can copy the DNA of a single cell Uses DNA polymerase from hot spring bacteria ( Thermus aquaticus ) Automated to produce millions of copies in few hours.

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200 views • 5 slides

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