Lez 11. Regolazione post-trascrizionale Alternative splicing Regolazione di RNA terminazione RNA editing Trasposto nucleare localizzazione mRNA Fosforilazione di Initiation factors IRES Stabilità di mRNA Nonsense-mediated mRNA decay IRE/IRPs RNA silencing.By giza
Transcription. Transcription. Poly A. Poly A. Poly A. GFP. GFP. GFP. intron. PFG. gene silencing mRNA degradation. Translation. DICER. GFP. RISC. PFG. Poly A. CAP. CAP. CAP. CAP. CAP. Poly A. DICER. GFP. p 35S. p 35S. GFP. intron. PFG. No Fluorescence. Fluorescence.By neith
Suppl. F. Vec ShcC. EGF 100ng/ml 10min. - + - +. WB: a pTyr. Phosphorylation of ShcA by EGF is suppressed in the cells transiently expressing ShcC.By nate
Production of Human Immunoglobulin G Antibodies in Plants. A comparision of stable and transient expressions Amanda Forni. Plantibodies. Agrobacterium- mediated transformation Transient transgenics Particle bombardment Simultaneous introduction of multiple constructs Stable transgenics.By octavia-mccall
Custom Antibody Sequencing - Discovery of the CenturyBy katemeddy04
Commonly-used recombinant protein expression systems include bacterial, yeast, insect, and mammalian cell culture systems. With the rapid development of plant transgenic technology, the plant expression system has been widely applied in both agricultural and plant biological studies. It has been reported that many biological products (such as bioactive peptides, therapeutic proteins) were generated from plant systems such as tobacco, tomato, rice and lettuce. Transient expression of target proteins in the plant systems is realized by agroinfiltration of plant tissues. Following successful agrobacterial transformation, target recombinant proteins, such as antibodies, vaccines, and enzymes can be expressed in high amount within the plant system. Comparing to the traditional bacterial, yeast, or mammalian expression systems, the plant expression system has the advantages of higher protein yield, lower cost, shorter turn-over time and increased safety. Among all plant species, the lettuce transient expression system is considered as the most promising system for the expression of recombinant proteins.By Anserj339
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