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Single-molecule detection of DNA transcription and replication

Single-molecule detection of DNA transcription and replication. Transcription initiation by RNA polymerase. D Wr = +1. promoter. RNAP. Topology of promoter unwinding. Lk = Tw + Wr = const. D Tw = -1. Observation of promoter unwinding by bacterial RNA polymerase. Negatively supercoiled DNA.

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Twait tunwound - PowerPoint PPT Presentation

Single-molecule detection of DNA transcription and replication