Whole cell lysate - PowerPoint PPT Presentation

HOW MASS SPECTROMETRY CAN IMPROVE YOUR RESEARCH

HOW MASS SPECTROMETRY CAN IMPROVE YOUR RESEARCH. http://biosciences.exeter.ac.uk/facilities/spectrometry/. Hannah Florance H.V.Florance@exeter.ac.uk bs-mass-spec@exeter.ac.uk. The University of Exeter Science Strategy – Systems Biology. 13.30 Hannah Florance

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i.p : GFP- Tspan blot: GFP- Tspan

Tspan14. Tspan12. CD9. -. GFP- Tspan :. +. +. +. +. HA-ADAM10:. A. i.p : GFP- Tspan blot: HA-ADAM10. **. 125. 80. 80. 100. HA-ADAM10 quantitation from GFP i.p . Relative ADAM10 level. 75. 58. 58. 58. 50. 25. 0. B. whole cell lysate blot: HA-ADAM10. **. 60. 40.

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IgG

c-SRC. LCK. LYN. HCK. FYN. IgG. phospho-SFK. β -actin. Supplemental Figure 1. Lyn is the predominant activated SFK in AML

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158 views • 1 slides

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