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-200 pb

A. CpG site. 8. 7. 6. 5. 4. 3. 2. 1. +1. XAF1 gene. -200 pb . -100 pb. P2. P1. 1. 2. 3. 4. 5. 6. 7. 8. CpG Site:. B. Control-1. Control-2. SF295. NT. Glioblastoma. SF539. IFN– b 24 h. Cervical carcinoma. HeLa. Neuroblastoma. SK-N-AS. C. E. 100. SF295.

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-200 pb

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  1. A CpG site 8 7 6 5 4 3 2 1 +1 XAF1 gene -200 pb -100 pb P2 P1 1 2 3 4 5 6 7 8 CpG Site: B Control-1 Control-2 SF295 NT Glioblastoma SF539 IFN–b 24 h Cervical carcinoma HeLa Neuroblastoma SK-N-AS C E 100 SF295 SF539 80 NT 24h NT 24h 60 Average methylation Within XAF1 promoter (%) XAF1 40 SF295 SF539 20 b-actin 0 SK-N-AS HeLa SF295 SF539 Control NT IFN D F 5-AZA-dC 5-AZA-dC 3500 NT IFN 20nM 50 nM 100nM 20nM 50 nM 100nM 3000 XAF1 SF295 2500 b-actin Fold XAF1 transcript induction (XAF1/GAPDH) 2000 1500 XAF1 HeLa 1000 b-actin 500 0 SK-N-AS HeLa

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