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Methylation Patterns of XAF1 Gene Promoter in Glioblastoma and Cervical Carcinoma Cells

Investigating the average methylation levels within the XAF1 gene promoter through CpG sites in various cell lines including SF295, SF539, HeLa, and SK-N-AS. Analysis of XAF1 transcript induction in response to IFN-β and 5-AZA-dC treatments.

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Methylation Patterns of XAF1 Gene Promoter in Glioblastoma and Cervical Carcinoma Cells

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  1. A CpG site 8 7 6 5 4 3 2 1 +1 XAF1 gene -200 pb -100 pb P2 P1 1 2 3 4 5 6 7 8 CpG Site: B Control-1 Control-2 SF295 NT Glioblastoma SF539 IFN–b 24 h Cervical carcinoma HeLa Neuroblastoma SK-N-AS C E 100 SF295 SF539 80 NT 24h NT 24h 60 Average methylation Within XAF1 promoter (%) XAF1 40 SF295 SF539 20 b-actin 0 SK-N-AS HeLa SF295 SF539 Control NT IFN D F 5-AZA-dC 5-AZA-dC 3500 NT IFN 20nM 50 nM 100nM 20nM 50 nM 100nM 3000 XAF1 SF295 2500 b-actin Fold XAF1 transcript induction (XAF1/GAPDH) 2000 1500 XAF1 HeLa 1000 b-actin 500 0 SK-N-AS HeLa

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