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Metody fluorescence chlorofylu Jaro 2014. Fotoinhibice fotosyntézy fluorometrické metody stanovení. M. Barták. http://www.esf.edu/efb/schulz/Limnology/phytoecol1.html. http://www.jeffs251lab.com/category/lab4/. Basic de-excitation mechanisms of Chl a.
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Metody fluorescence chlorofylu Jaro 2014 Fotoinhibice fotosyntézyfluorometrické metody stanovení M. Barták
Basic de-excitation mechanisms of Chl a Photosynth.process (linear transport e- ) Thermal dissipation Chl a* Regulation between PSII and PSI Chlorophyll fluorescence
Photosynthetic pathway Reversible transport Qa 3(P680+ Pheo-) 1(P680+ Pheo-) 3 P680* 1 P680* 3Bcarotene* P 680 Fluorescence O2* 3Bcarotene* Triplet Recomb. Singlet Recombin. Charge separation heat 3Chla* Chla* heat Antennae q.
Metody stanovení míry fotoinhibice • Kautského křivka fluorescence chlorofylu doplněná o zhášecí mechanismy • Časové řady parametrů fluorescence chlorofylu
qP = (Fm’- F) / (Fm’-Fo’) NPQ = (Fm - Fm’) / (Fm’) qN = qE + qT + qI qE
qT = Fm’ after rapid relaxation is complete usually with the actinic light turned off usually one hour - Fm’ at qE /Fm’ at steady state. qI = Fm-Fm’ at qT/ Fm’ at steady state. qN = Fm - Fm’/ Fm-Fo qL = qP(Fo’/F’) Y(NO) = 1/NPQ +1 + qL((Fm/Fo)-1) Y(NPQ) = 1 - Y - Y(NO) 1 = qL + Y(NPQ) + Y(NO) qP = (Fm’- F) / (Fm’-Fo’) NPQ = (Fm - Fm’) / (Fm’) qN = qE + qT + qI qE http://www.optisci.com/cf.htm
http://www.biol.s.u-tokyo.ac.jp/users/seitaipl/personal/terashima/terashima_e.htmlhttp://www.biol.s.u-tokyo.ac.jp/users/seitaipl/personal/terashima/terashima_e.html
Brno, Laboratoř fotosyntetických procesů Senzitvita vůči dlouhotrvající fotoinhibici A zotavení se v