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Analysis of Glucose Concentration in Yeast Fermentation: Advanced Genetic Constructs

This study investigates glucose concentration variations in yeast fermentation, focusing on specific genetic constructs of *Saccharomyces cerevisiae*. Using different strains, including laboratory (W3031a) and industrial (GA001, Fleischman, and others), we analyze outcomes in uniform two-phase systems with and without glucose. The research encompasses PCR amplification techniques of the FLO10 gene and its impact on fermentation efficiency. Results highlight the importance of specific genetic configurations and fermentation conditions in maximizing glucose utilization and overall yeast performance.

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Analysis of Glucose Concentration in Yeast Fermentation: Advanced Genetic Constructs

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  1. Fleischman Selvagem FleischmanTransformada Fleischman Selvagem FleischmanTransformada Duas fases (Fase superior ainda turva) Uniforme Duas fases Uniforme SEM GLICOSE COM GLICOSE 1-Lambda Hind III 2-FLO10 amplificado da linhagem laboratorial W3031a 3-FLO10 amplificado da linhagem industrial GA001 4-FLO10 amplificado da linhagem industrial Fleischman FLO10 amplificado da linhagem industrial Pedra2 Amplificação do FLO10

  2. YEp 352 (A) YEp352-FLO5 (B) A. B. C. YEp352 YEp352 pYADE4 YEp-FLO5 pUC-BB MOX-BB ADH2 FLO5 pYADE4-FLO5 (C) NI NI FLO5 FLO5 MOXBB-FLO5 MOXBB-FLO5 Determination of glucose concentration (E) SacI SmaI (SphI) ClaI ClaI SacI HincII 12 14 16 18 20 22 12 14 16 18 20 22 12 14 16 18 20 22 12 14 16 18 20 22 SacI SmaI/HincII pYADE4-FLO5 YEp352-MOXBB-FLO5 YEp352-BB-FLO5 (D) pYADE4 pGEM-T-FLO10 ADH2 FLO5 BamH I Sal I BamH I Sal I BamH I Sal I pYADE4-FLO10 (SphI)/SmaI ClaI

  3. YEp 352 (A) YEp352-FLO5 (B) pYADE4-FLO5 (C) Determinação da concentração de glicose (E) 12 14 16 18 20 22 12 14 16 18 20 22 12 14 16 18 20 22 12 14 16 18 20 22 2,5 2 1,5 YEp352-BB-FLO5 (D) (%) Concentração de glicose 1 0,5 0 0 10 20 30 -0,5 Tempo (h) YEP352-FLO1 YEp352 pYADE4-FLO1 YEP352-FLO1-BB

  4. ESTÉRIL Pré inóculo S. cerevisiae 50 l 300 l 12.000 l 150.000 l Vinho para a destilaria INÍCIO DE PRODUÇÃO MOSTO * * * * * * * Centifugação 1° estágio 2° estágio 3° estágio 4° estágio 400.000 litros por dorna TRATAMENTO

  5. pYADE4-FLO5 ADH2 FLO5 PCR CAN1 ADH2 FLO5 CAN1 Crossing Gene CAN1 no cromossomo Construção se integra no cromossomo CAN1 ADH2 FLO5 CAN1

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