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OVERVIEW R&D, Laboratories and Analysis Services Gastien Godin Jacques Gagnon, PhD

OVERVIEW R&D, Laboratories and Analysis Services Gastien Godin Jacques Gagnon, PhD General Manager Scientific Director Fishery and Marine Coproducts Division Fredericton , NB October 8th, 2014. COASTAL ZONES RESEARCH INSTITUTE (CZRI). CZRI Highlights – Value- A dded F ood S ector

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OVERVIEW R&D, Laboratories and Analysis Services Gastien Godin Jacques Gagnon, PhD

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  1. OVERVIEW R&D, Laboratories and Analysis Services Gastien Godin Jacques Gagnon, PhD General Manager Scientific Director Fishery and Marine Coproducts Division Fredericton , NB October 8th, 2014

  2. COASTAL ZONES RESEARCH INSTITUTE (CZRI) • CZRI Highlights – Value-AddedFood Sector • Mobile Pilot Unit LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE AQUACULTURE FISHERY AND MARINE COPRODUCTS LABORATORIES AND ANALYSIS SERVICES

  3. LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE Dr. Mathieu Quenum – Scientific Director Email : mathieu.quenum@irzc.umcs.ca Tel. : (506) 336-6600 Fields of Expertise and Strategic Research Concentration • Biomass & Bioenergy • Horticulture, Agriculture & Berries • Sustainable Production of Sphagnum & Added Value Products • Soil, Water & Environment • SustainableDevelopment in Coastal Areas

  4. AQUACULTURE Dr. André Dumas– Scientific Director Email : andré.dumas@irzc.umcs.ca Tel. : (506) 336-6600 Fields of Expertise • Nutrition of fish and crustaceans • Nutrition modelling • Arctic charr, salmon, oyster and lobster production • Product development and innovation management Research focus areas • Nutritional evaluation of commodity and innovative ingredients, prebiotics, probiotics, immunostimulants • Nutrigenomics

  5. FISHERY AND MARINE COPRODUCTS Dr. Jacques Gagnon– Scientific Director Email : jacques.gagnon@irzc.umcs.ca Tel. : (506) 336-6600 Fields of Expertise • Development of green processes • Lab scale spaces with hydrolysis, drying & centrifugation capacities • 53’ pilot multifunctional unit for turnkey project on site • Product development and innovation management Research focus areas • Valorization and commercialisation of marine coproducts • Natural health products

  6. LABORATORIES AND ANALYSIS SERVICES • Chemical/microbiological analyses • Environmental, nutritional, etc. • Food safety • ISO/IEC 17025 (CALA) • R&D support • Fattyacids & pigments • Aminoacids (in development) • Personnalizedservices • On-site, technical, process design, etc. • Regulatory support, nutritional labelling, shelf life • Coproductsrecovery and characterization

  7. Mobile Pilot Unit • Various tanks (2 x 1500 liters) • 8 x 100 liters double walls for temperature control • Variouspumps • Filtration unit (sweco) • Mixers • Etc. • 53 feetreefertrailer • Processing room withtemp. control • Walk-in cold room and freezer • 600, 220, 110 volts connections OPEN FOR BUSINESS We look forward to new collaboration opportunities!

  8. Highlights – Value-Added Food Sector “Growing Our Industry: Making history with the new biotechnologies” Acadian Peninsula Convention Centre, Shippagan, NB May 21-23, 2014 www.shippagan.ca CZRI co-hosted (with AFBPA) the Sixth Acadian Peninsula Food Processing Conference

  9. MAXIMIZING THE VALUE OF MARINE COPRODUCTS: APPLICATIONS IN NUTRISCIENCES Jacques Gagnon, PhD and colls. Fisheries Council of Canada 2014 Conference October 8th

  10. Valorization of effluents, residues and marine coproducts VALEUR.COM • VALEUR.COM = VALorisation desEffluentsd'Usine, des Résidus et des COproduitsMarins) • Full title –AIF project: • DEVELOPMENT AND COMMERCIALIZATION OF MARINE COPRODUCTS FOR USE IN ANIMAL AND HUMAN NUTRITION AND ITS APPLICATION TO THE PREVENTION OF OBESITY, DIABETES AND NEURODEGENERESCENCE • CZRI research team: Nadia Tchoukanova • Sébastien Plante and Jacques Gagnon

  11. BioAtlantech Survey (Biosciences) • Geographical clustering aquaculture and marine • St. George/St. Andrews (7 Companies + 2 Research Institutions) • Acadian Peninsula (7 Companies + 1 Research Institution) Now BioNB, 2013

  12. LIST OF COPRODUCTS

  13. SEA, PROCESSING AND AQUAFEED: A VALUE CREATION PATHWAY OF SEAFOOD RAW MATERIALS André Dumas, PhD & colls The Fisheries Council of Canada 2014 conference

  14. Aquaculture in the World Source: FAO (2012)

  15. Aquafeeds in the World • 20 to 23 millions MT aquafeed/yr (2006) • ~3.7 million MT/yrfish meal (FM) = $5.5 bln • ~0,8 million MT/yr of fish oil (FO) = $1.0 bln Source: Tacon & Metian (2008)

  16. FM: source of beneficial nutrients • Essential amino acids (AA) • Valuable AA: Taurine, hydroxyproline • Vitamins (e.g. B12) • Minerals (e.g. available P) FO: source of beneficial fatty acids • Omega-3 to meet consumer expectations

  17. FM & FO cost Source: Tacon & Metian (2008)

  18. Alternatives to FM & FO: what does the sea have to offer? • Seafood processing by-products from sustainably managed fisheries • New business opportunities

  19. Shrimp processing 30% Residues (70%) Liquid Solids Recycled Photos: http://dailyfoodporn.wordpress.com/tag/nordic-shrimp/

  20. Shrimp residue meal

  21. Shrimp residue meal

  22. Shrimp residue meal

  23. Shrimp residue meal (SRM)

  24. CONCLUSION • 1. SRM: • Candidate alternative to $$$ sources of beneficial nutrients • 2. Next steps • Approval by regulatory agencies

  25. LABORATORIES AND ANALYSIS SERVICES:BIOCHEMICAL CHARACTERIZATION OF MARINE PRODUCTS AND COPRODUCTS Josée Boudreau, PhD, Marie-Hélène Thibault, PhD, Claude Pelletier, M.Sc, Mathieu Ferron, M.SC

  26. MARINE PROCESSING COPRODUCTS Over the years, ourmultidisciplinary team has developped an expertise in the screening and characterization of several marine processingcoproductssuch as: Seacucumber Lobster Snow crab • Mouth • Anus • Viscera • Head • Hepatopancreas • Shell Arcticshrimp Herring • Cephalothorax • Shell • Proteinwaste • Head • Tail • Viscera • Milt • Roe

  27. CHARACTERIZATION OF MARINE COPRODUCTS In a first step, proximateanalysis, usingstandard methods, reveals the major constituents in the productsunderstudy Immature herringroe Herring milt

  28. CHARACTERIZATION OF MARINE COPRODUCTS Each class of compounds can be probed further to gain knowledge about the product at hand and the biomolecules it contains Immature herringroe • Marine products are known to berich in phospholipids and omega-3 fattyacids, as well as fat-soluble biomoleculessuch as vitamins(A, D, E) and pigments • Lipids are thereforeoften one of the main focuses in the study of marine coproducts

  29. MARINE COPRODUCT LIPIDS Each Lipid classes Fattyacid profile • Phospholipids • Sterols • Triglycerides • Wax esters • Neutralvs polar lipids • Omega 3 • Omega 6 • EPA, DHA • Saturated • Mono-, poly-unsaturated Rancidity/oxidation Other bioactive molecules • Free fattyacids (FFA) • Peroxide value • p-anisidine value • Vitamins (A, D and E) • Carotenoid pigments (Astaxanthin, β-carotene, etc.)

  30. MARINE COPRODUCT PIGMENTS Current projects in our laboratories include the detailed study and characterization of astaxanthin, a widespread and commercially relevant marine pigment, in shellfish processing coproducts. • For animals: • Widely used in aquaculture for flesh pigmentation improved fish growth • For humans: • Powerful antioxidant • Anti-tumor • Anti-inflammatory • Protects against cardiovascular diseases and ulcers • Treats neurodegenerative diseases Astaxanthin

  31. ASTAXANTHIN ANALYSIS Various chemical forms of astaxanthin naturally occur and influence its biological properties and stability. Different analytical methods are required to study these complex molecules. Astaxanthin 1. Free or withfattyacid(s) Free, monoester or diester 2. Geometricalisomers All trans, 9-cis, 13-cis, etc. 3. Optical isomers {R,R’}, {R,S’} (MESO), {S,S’}

  32. ASTAXANTHIN ANALYSIS Current analytical capabilities in our laboratories include: • Quantification of free, esterified and totalastaxanthin • Identification of geometrical isomers (all trans, 9-cis, 13-cis, etc.) Work is in progress to develop a rapid, simple, in situ method for the identification of astaxanthin optical isomers in different products • High performance liquidchromatography (HPLC) • Normal phase • Reversed phase • Chiral reversed phase • RAMAN and FT-Infraredmicrospectroscopy • Microscope for in situimaging • « Same spot » technology for heterogeneoussamplemapping

  33. Fisherycoproductas nutraceutics (naturalhealthproductsand functionalfoods): Innovation basedtechnology Neurodegeneration • Anti-diabetes • Anti-inflammatory • Anti-obesity • Neuroprotection Diabetes Insulin resistance Obesity Inflammation

  34. Valorization of Coproductsfromfishprocessing plants CZRI Purification Analyses Processdevelopment Pilot scale unit NRC- Halifax Bioguidedfractionation Purification Adipocytes cell model (Obesity) NRC- Charlottetown in vitro and in vivo diabetes model Bioguidedfractionation Dalhousie University in vitro and in vivo Alzheimer model Jacques Gagnon

  35. New, added-value products Product development CZRI phases • Fish cuttings • Shrimp • Sea cucumbers Aquaculture or marine biomass • Animal feed • Natural health products (NHPs) • Human food products Chemical analysis • Natural product chemistry extraction • Chemical fractionation • Chemical characterization CZRI Bio-analysis • In vitro bioassays • In vivo models Lead bioactives • Product formulation • Product stability • Market opportunities • Scale up • Food technology centers • Partnering initiatives

  36. Summary • Extracts and protein hydrolysates prepared: ~100 • Focus on lead products that would be beneficial in metabolics disorders (e.g. pre-diabetes, obesity), be neuroprotective, or anti-inflammatory • Shrimp extracts and herring milt hydrolysatesgave good activity for antidiabetic and neuroprotective effects • These should be further assessed by the development of health supplement ingredients with animal model of alzheimer’s disease and diabetes Jacques Gagnon

  37. Funding Agencies • Atlantic Canada Opportunities Agencies (ACOA)- Atlantic Innovation Fund (AIF) • New Brunswick Innovation Fund (NBIF) • Industrial Partners: ex: Island Fishermen Cooperative Association • New BrunswickDepartment of Agriculture, Aquaculture and Fisheries Jacques Gagnon

  38. Whatisin the pipeline • Patent published… more to come withantidiabeticactivities of shrimpoil • Marketfeasabilitystudywithshrimpoil in progress • Animal model of diabetes and neurodegenerationwithshrimpoil and herringmilthydrolysate to confirm positive effects are in progress • Looking for a commercial receptorpartner • Data collection in animal models to support a humanclinical trial withshrimpoil (NHP) • Purification processofshrimpoil for human use at labscale- withscaling up at industriallevel Jacques Gagnon

  39. Questions? THANK YOU

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