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Effects of VEGF on Endothelial Cell Migration in Monolayer and 3D Spheroid Cultures of CT26 Cells

This study investigates the concentration of VEGF in both untreated and treated CT26 cell cultures, focusing on its influence on endothelial cell migration. Utilizing monolayer and 3D spheroid conditions, we measure sICAM-1 and other adhesion molecules like LFA-1 and mICAM-1, dissecting their roles in cellular interaction during treatment. The findings aim to enhance understanding of cancer cell behavior and potential therapeutic targets in tumor microenvironments.

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Effects of VEGF on Endothelial Cell Migration in Monolayer and 3D Spheroid Cultures of CT26 Cells

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  1. B A 1000 # 800 600 * VEGF Concentration (pg/106 cells) ## 400 ** 200 0 Clx Clx Clx Clx Untreated Cells sICAM-1- Treated Cells Untreated Cells sICAM-1- Treated Cells Monolayer-Cultured CT26 3D-Spheroid- Cultured CT26 CT26 CELL COX 2 VEGF ENDOTHELIAL CELL MIGRATION sICAM-1 LFA-1 mICAM-1

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