1 / 16

Manganese Oxidizing Bacteria

Manganese Oxidizing Bacteria. Cynthia McClain HMC 2012 July 19, 2012. Mn(II ) Mn(III,IV ) Soluble. Mn-oxide/oxyhydroxide precipitate. Most MnOx in nature formed biotically ! . Niche. Physical and chemical gradients. Manganese Oxidizing Bacteria.

solada
Télécharger la présentation

Manganese Oxidizing Bacteria

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Manganese Oxidizing Bacteria Cynthia McClain HMC 2012 July 19, 2012

  2. Mn(II) Mn(III,IV) Soluble Mn-oxide/oxyhydroxide precipitate Most MnOx in nature formed biotically!

  3. Niche Physical and chemical gradients

  4. Manganese Oxidizing Bacteria • Multicopperoxidase or peroxidase proteins

  5. Isolation Collect water or sediment sample (slurry) Streak 100-200µl supernatant on K media plate: MnCl2, peptone, yeast extract Incubate 1-3 weeks Restreak brown colonies Test with Leucoberbelin Blue (LBB)

  6. Experimental Results • Different morphologies • Color • Size • Positive LBB • Soil • Sand from mouth of Elkhorn Slough (2) • Kirby Park oily sheen (1) • Baby seal beach sand (1)

  7. Movie of LBB Test • Sand from mouth of Elkhorn Slough

  8. Soil

  9. Soil

  10. Kirby Park Oily Sheen

  11. Kirby Park Oily Sheen

  12. Baby Seal Beach

  13. Baby Seal Beach

  14. Manganese Oxidizing Bacteria • Phylogeny • Why oxidize Mn? • Environmental Significance

  15. Thank you!

More Related