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Basics Of Introduction, Chemistry, Protocols & Troubleshooting. DNA. Sequencing. Introduction to: Fluorescent Sequencing and Electrophoresis. Sanger Sequencing. Sanger Sequencing Basic idea. NH. NH. 2. 2. O. O. O. P. O. O. P. O. N. N. O. O. O. N. O. N. O. O. HO.
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Basics Of Introduction, Chemistry, Protocols & Troubleshooting DNA Sequencing
Introduction to:Fluorescent Sequencing and Electrophoresis Sanger Sequencing
NH NH 2 2 O O O P O O P O N N O O O N O N O O HO HO DIDEOXYRIBONUCLEIC ACID (ddNTP) terminator DEOXYRIBONUCLEIC ACID (dNTP) Sanger Sequencing Chemistry
Sanger Sequencing Basic idea w/ fluorescence dNTP ddNTP primer
ddATP A ddCTP AC ddGTP ACG ddTTP ACGT Chemistry overviewDye terminators +
Sanger Sequencing Cycle sequencing denaturation annealing rxn. mixture extension A CG T G A CG T A CG T labeled products A C G T T A A
Sanger Sequencing Cycle sequencing steps 50°C 50°C 96°C 60°C 10” 4’ 4’ 5” 4oC 8 x 25
0’ Molecules Separation Electrophoresis principle
30’ Molecules separation Electrophoresis principle
45’ Molecules separation Electrophoresis principle
90’ Molecules separation Electrophoresis principle
denaturation Sanger Sequencing Electrophoresis principle
Dr. Mohamed Abdel FattahApplication SpecialistMolecular BiologyAnalysisAB Co.Cairo, Egypt