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Dicer-Like

Dicer-Like. A RNA Helicase Gene Involved in Gene Silencing. UCSF. Max Bachour Jessica Chen Chris McQuilkin. A RNA Helicase Gene Involved in Gene Silencing. RNA helicase is an enzyme that unwinds RNA and modifies its structure

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Dicer-Like

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  1. Dicer-Like A RNA Helicase Gene Involved in Gene Silencing UCSF Max Bachour Jessica Chen Chris McQuilkin

  2. ARNA HelicaseGene Involved in Gene Silencing • RNA helicase is an enzyme that unwinds RNA and modifies its structure • Involved in transcription, splicing, translation, and gene silencing

  3. A RNA Helicase Gene Involved inGene Silencing How does Dicer-Like Help with Gene Silencing?

  4. RNAinterference • Dicer and Dicer-Like (DCL) enzymes are involved in RNA interference (RNAi) • Nontranslated RNA fragments bind to mRNA and prevent translation into a protein

  5. Evolutionary Origins • A defense system for eukaryotes against double stranded viruses and transposons

  6. What is Dicer’s role in RNAi? • Activated by exogenous double-stranded (ds) RNA • miRNA (micro RNA) -small, non-coding regions of double-stranded (ds) RNA 21-22 nucleotides

  7. What is Dicer’s role in RNAi? • siRNA (small interfering RNA) non-coding regions of dsRNA 20-25 nucleotides in length

  8. What Happened When the Dicer-Like Gene Was Knocked-out? Seed from a Wild Type Plant

  9. Dicer-Like Mutants Cone Shaped Suspensor Cone Shaped Suspensor

  10. How did the Dicer Knock-out Result in the Cone Shape? • Knocking-out the dicer gene keeps RNAi from forming • This keeps certain genes in the seed from being inhibited. • The lack of inhibition caused the cells in the embryo to multiply irregularly

  11. Why did we knock-out dicer? • Provided a positive control to compare to our T-DNA knock-out line • Made sure that negative results weren’t due to technical failure • Our main goal is to discover the function genes in arabidopsis.

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