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Production of clean seed

Y Y Y Y Y. Y Oe Y Oe Y Oe Y Oe Y Oe. Y Oe Y Oe Y Oe Y Oe Y Oe. Y O Y O Y O Y O Y O. Production of clean seed. The virus-free program at WRPIS (Pullman, WA).

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Production of clean seed

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  1. Y Y Y YY YOe YOe YOe YOe YOe YOe YOe YOe YOe YOe YO YO YO YO YO Production of clean seed The virus-free program at WRPIS (Pullman, WA)

  2. 1) History2) The “TEST”3) The “clean-up”4) The future

  3. History • 1983 >70% infected w/ BCMV • 1985 original protocol • 1994 leaf tissue testing • 23.3% of collection virus-free

  4. The “TEST” • Tissue harvest • ELISA testing • Clean  • “Dirty” 

  5. The “clean up” • <10% BCMV infection • rouging • >10% BCMV infection • single planting, harvest & testing • Re-combining clean seed

  6. ¿ The future ? • Widely recognized protocol • Adapted by other germplasm collections • Virus testing technician • Part-time help? Soft money? • Suggestions?????

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