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Bioreactors:

Bioreactors:. Presented by: Shehneela Baseer 117113 Zainab Sajjad 117114. Contents. Introduction to Bioreactors Types of Bioreactor designs Conclusion. Bioreactors. Any manufactured or engineered device or system that supports a biologically active environment.

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Bioreactors:

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  1. Bioreactors: Presented by: Shehneela Baseer 117113 Zainab Sajjad 117114

  2. Contents • Introduction to Bioreactors • Types of Bioreactor designs • Conclusion

  3. Bioreactors • Any manufactured or engineered device or system that supports a biologically active environment

  4. Types of Bioreactor designs • Stirred tank reactors • Bubble-column reactors • Air lift reactors • Drum rotating reactors • Immobilized plane cell reactors • Membrane reactors

  5. Stirred tank reactors • Air is dispersed by mechanical agitation.

  6. Advantages • Better control over the environment of the culture.

  7. Disadvantages • Can cause damage to the cells • High energy demand • Complexity in construction • Difficult to scale up.

  8. Bubble-column reactors • One of the simplest type of gas – liquid bioreactors.

  9. Advantages • Facilitates sterile operation • Less damaging to shear-sensitive cells • Scale up is relatively easy.

  10. Disadvantages • Undefined fluid flow pattern inside the reactor. • Non-uniform mixing.

  11. Air-lift reactors • Works on draught tube principle.

  12. Advantages • Reasonable mixing with low shear • Operating cost is low. • Less contamination

  13. Disadvantages • Insufficient mixing at high cell densities.

  14. Drum rotating reactors • Consists of horizontally rotating-drum on rollers connected to a motor.

  15. Advantages • High oxygen transfer. • Good mixing • Facilitated better growth and impart less hydrodynamic stress.

  16. Disadvantages • Difficult to scale up.

  17. Immobilized plane cell reactors • Immobilization of plant cell into a suitable carriers. • Either in natural (alginate, agar) or synthetic (polyacrylamide)

  18. Membrane reactors • Cells are separated from growth medium by membrane

  19. Advantages • Environment is more easily controlled • Better control over cell density.

  20. Example: • Insulin Bioreactor Design

  21. Reactor Considerations: • Production of insulin precursor • 5000 kg insulin per year • Assumes 20 % loss due to purification • kLa within 10% of 2088 hr -1 • Prevents oxygen limited reaction • Prevents anaerobic metabolism • Glucose concentration < 0.5 g/L • Prevents formation of ethanol • CSTR configuration • Jacket heat exchanger • Price

  22. How Unit Operates • Substrate feed • Glucose, ammonia, mineral salts • Cellular metabolism of substrate • Extracellular production of insulin • Air sparging for oxygen delivery • Impellers for mixing of nutrients and oxygen

  23. Conclusion • Hence, with the help of different types of bioreactors, commercial production of secondary metabolites is not only possible but also profitable.

  24. Thank you !

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