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Resection of DSBs from Sparsely and Heavily Ionizing Radiation in Different Cell Cycle Phases

This study investigates the resection of double-strand breaks (DSBs) caused by sparsely ionizing radiation, which are resected in the G2 phase, compared to more complex DSBs caused by heavy ion radiation, which are repaired in both G1 and G2 phases. NFFhTERT cells were irradiated, and DSB resection analyzed two hours post-irradiation for G1 and S/G2 phases. The results indicate that X-ray irradiated G1 cells show no significant number of RPA positive cells, evidenced by statistical analysis of independent experiments.

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Resection of DSBs from Sparsely and Heavily Ionizing Radiation in Different Cell Cycle Phases

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  1. SI Figure S4. DSBs caused by sparsely ionizing radiation are resected in G2-phase; more complex DSBs caused by heavy ion radiation are resected in G1 as well. NFFhTERT cells were irradiated and treated as in Figure 3 A. DSB resection (RPA) was analyzed 2 h after irradiation for G1- (CENP-F negative) as well as S/G2-cells (CENP-F positive). Error bars: X-ray experiment: SEM of three independent experiments; carbon ion experiment: binomial error of the mean of one experiment. At least 30 G1 and S/G2 cells were analyzed. The data for X-ray irradiated G1 cells does not show a significant number of RPA positive cells (Fisher's exact test).

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