Supplementary figure. 1 Ashino et al. 6 ■ CCR6 4 Relative expression of mRNA versus b-actin 2 0 Th17 polarized cells Naive Th cells Naive Th cells Th17 polarized cells DO11.10 Tg IL-17-/- DO11.10 Tg Supplementary Figure 1 CCR6 mRNA levels are not changed between wild type Th17 and IL17-/- Th17 polarized cell population. CD4+CD45RB+ naïve Th cells were isolated from DO11.10 Tg and IL-17-/- DO11.10 Tg mouse spleen cells. Isolated naive Th cells were cultured in Th17 condition for 8 days. Naive Th cells and Th17 polarized cell population derived from DO11.10 Tg and IL-17-/- DO11.10 Tg were subjected to real-time PCR analysis. One experimental group was composed of three culture wells (n=3).