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Microscope Notes

Microscope Notes. Learning how to use the microscope. Different Kinds of Microscopes. There are 2 kinds of microscopes:. Simple: one lens ( magnifying glass) Compound: 2 or more lenses. Parts of the Microscope. 1. Revolving Nosepiece. Parts of the Microscope. 2. Lens/Objective.

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Microscope Notes

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  1. Microscope Notes Learning how to use the microscope Different Kinds of Microscopes

  2. There are 2 kinds of microscopes: Simple: one lens (magnifying glass) Compound: 2 or more lenses

  3. Parts of the Microscope 1. Revolving Nosepiece

  4. Parts of the Microscope 2. Lens/Objective

  5. Parts of the Microscope 3. Stage

  6. Parts of the Microscope 4. Disc Diaphragm (controls amount of light) 1 = lowestsetting/ 5 = highest setting

  7. Parts of the Microscope 5. Light source

  8. Parts of the Microscope 6. Base

  9. Parts of the Microscope 7. Eyepiece

  10. Parts of the Microscope 8. Arm

  11. Parts of the Microscope 9. Stage Clips

  12. Parts of the Microscope 10. Course Focus/ Adjustment

  13. Parts of the Microscope 11. Fine Focus/ Adjustment

  14. Calculating Magnification Multiply lens powers together!

  15. Eyepiece: 10 Xby itself • Low Power: 4X; red color; and a TOTAL magnification of 40 X mainly used for large objects/scanning • Medium Power: 10 X, yellow color; TOTAL magnification of 100 X • High Power: 40 X;blue color; and a TOTAL magnification of 400 X; mainly used for smaller objects/details

  16. When you are ready to start: • Make sure it is on LOW power! • The stage should be all the way down (you focus by slowly moving it up!) • Check the light setting (should be at 1-2 to start)

  17. Other Things to Consider: Carrying the Microscope: Always use 2 hands!

  18. Other Things to Consider: Never use the COURSE FOCUS KNOB while on HIGH power.

  19. When putting the microscope away (at the end of the day): • It should be on LOW power. • The cord should be WRAPPED around the base. • The stage should be all the way DOWN (do this with the course focus knob!) • The light switch should be OFF

  20. TROUBLESHOOTINGWhat if you can’t get your object into focus? • Check that you have one of the lenses clicked into place • Check that your disc diaphragm is in place (not IN BETWEEN settings)

  21. TROUBLESHOOTINGWhat if you can’t get your object into focus? • Clean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!) • GO BACK TO LOW POWER AND START OVER!

  22. Electron Microscopes • Use a MAGNETIC field to bend beams of light; used to see things at much higher level of detail • Can magnify images up to 1,000,000 times!

  23. Electron Microscopes There are 2 kinds: • SCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACES of objects

  24. Electron Microscopes There are 2 kinds: • TRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGH an object.

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