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Wednesday July 11. 2012 Cheryl Jennings, Bria Dawson, Even Skjervold , & Kali Luffy

Periorbital Drug Delivery. Wednesday July 11. 2012 Cheryl Jennings, Bria Dawson, Even Skjervold , & Kali Luffy. Overview. Continuing drug release study with FDA Started drug release study with Valganciclovir & Ganciclovir Visited the HIV clinic and met with Dr. Rupesh Agrawal

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Wednesday July 11. 2012 Cheryl Jennings, Bria Dawson, Even Skjervold , & Kali Luffy

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  1. Periorbital Drug Delivery Wednesday July 11. 2012 Cheryl Jennings, Bria Dawson, Even Skjervold, & Kali Luffy

  2. Overview • Continuing drug release study with FDA • Started drug release study with Valganciclovir & Ganciclovir • Visited the HIV clinic and met with Dr. RupeshAgrawal • Continued trying to find a common solvent

  3. Brief Drug Release Procedure • Remove 20uL from master plate into read plate with 180uL of 0.1M NaOH (for fluorescence) • Remove rest of PBS tween 80 from master plate and replace with 200uL of new PBS tween 80 • Put master plates back in oven to incubate • For dilution, we removed 20uL from the read plate and 180uL of 0.1M NaOH • Fluorescence readings of both read plates were performed and analyzed

  4. Example 96 Well Plates with Films

  5. FDA Release Study • Have had to 10X dilute every time point (we are currently on day 16) • This concludes that the amount of drug in the film is too great and could be decreased in future studies • Possible sources of error • Reusing well plates • Working under lighted conditions • Will continue this study until day 30 • Then analyze on “Master Sheet”

  6. Valganciclovir & Ganciclovir Release Study • Started the drug release study using 180 ulHCl buffer (pH 1) and 20 ul master plate soln. • Cannot perform fluorescence readings due to lack of calibration curves • Possible sources of error • Since not soluble in PBS drug release study may be fallible • Reuse of 96 well plates

  7. Solvent Solubility Tests • Qualitative testing using: • 0.2 g PCL (in 2 ml) overnight • 5 mg drug (in 1 ml) with and without heat

  8. The Solution • Tests were performed for 0.4 g PCL and 0.3+0.1 g PLGA+PCL in DMSO and DMF in preparation for a possible «Plan B» for casting • Vials were left stirring overnight, with some immediately encouraging results • DMF dissolved PCL at 50oC, while DMSO-PCL only melted to the side of the vial

  9. Friday, July 6th @ 2pm • Went to Communicable Disease Center 1 and met with Dr. RupeshAgrawal • Watched him perform routine follow up eye examinations • Took pictures of HIV patients for a new study he is working on • Watched 2 patients receive ocular injection • Patients receive 8 doses of 2000ug for 4 weeks and 4 doses of 1000ug the next 4 weeks • Drug is suspended in saline solution

  10. Next Steps • Continue release studies of both FDA, Ganciclovir & Valganciclovir • Find a solvent to dissolve Gan and PCL • Can make better films for both Ganciclovir & Valganciclovir • Complete calibration curves for both drugs • Use curves to find the best gains for drug release study to be read at

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