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Investigation of RhoGEF P115 Mutants and Their Interactions with Rac and PAK Proteins

This study explores the functional characterization of the RhoGEF P115, focusing on mutant forms including ∆N and ∆C variants. Using various techniques such as Western blotting (WB) and immunoprecipitation (IP-myc), we analyze the interactions of P115 with Rac and PAK proteins. The study aims to elucidate the role of specific domains of P115 in signaling pathways critical for cellular processes. Findings contribute to a deeper understanding of the mechanisms involved in Rho family GEF signaling.

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Investigation of RhoGEF P115 Mutants and Their Interactions with Rac and PAK Proteins

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  1. A B p115RhoGEF P115 ∆N ∆C C-only RGL DH PH P115 1 912 Rac + + + + + ∆C + + + + + + + + + PAK 800 1 201 ∆N 351 912 127 DHPH 351 771 80 C-only 771 912 IP-myc WB AU1 C P115 ∆N P115 DHPH PRG DHPH Larg DHPH 31 Rac + + + + + PAK + + + 201 127 IP-myc WB AU1 80 TCL WB AU1 TCL WB AU1 31

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