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Learn about the essential aspects of autopsies, including when they are required and the procedures involved. Discover the role of forensic pathologists, the process of examining inner organs, and what evidence is collected during an autopsy. Explore the importance of toxicology, histology, and serology in determining the cause of death. Find out how blood, hair, tissues, and other samples are analyzed to provide valuable insights. Practice virtual autopsies online and dive into biomolecules like carbohydrates, proteins, lipids, and nucleic acids.
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Intro By law, deaths that occur under the following circumstances must be investigated: • Violent crime, suicide, or accident • Within 24 hours of entering a hospital or as a result of surgery • A natural death when a doctor is not present or the patient is not under the care of a medical facility • Occurs in police custody or in a correctional facility • Results from a communicable disease that may pose a threat to public health
Autopsy Basics • Performed by a forensic pathologist – a medical doctor who is trained to identify why and how someone died and present those finding in court. • At the scene – Postmortem interval is estimated (amount of time since death) • In the ME’s office: • Determine cause, manner & mechanism of death
Procedure 1) A Y-incision is made on the torso to expose the inner organs
Procedure • Each organ is removed, weighed, carefully observed, and a sample is taken and sent to appropriate people • Toxicology • Histology • Neuropathology • Serology
What to look for: • Blood for DNA – ME’s collect blood from arteries for identification and toxicology (determines drug & alcohol content). Dried blood on skin could be from perpetrator • Semen – If sexual assault is suspected • Hair – a “control sample” is collected to compare to unknown hairs found at the scene • Tissue – Tissue samples are sent to histology for analysis • Fingernails – Could contain DNA from perpetrator if a violent crime • Bite Marks – Images sent to forensic odontologists
Close Up • Everything is put back in place and a “baseball stitch” Is used to close back up. • A report is made….. And yes…. They have to use complete sentences and punctuation counts.
Now you try! • Go to: http://australianmuseum.net.au/interactive-tools/autopsy/ - You must use internet explorer! (http://bit.do/GH6 ) Describe in 8-10 sentences the procedure of an autopsy. Points counted off for missing sentences or short sentences obviously used to fill space.
Now you try! • Go to: http://webcom4.grtxle.com/khforensics/VirtualAutopsyPackage/VirtualAutopsy3_fs.html ( http://bit.do/GJf ) Do 2 autopsies then have the program email me the results: Cassie_Torres@RoundRockISD.org
Biomolecules Review! • Carbohydrate • Proteins • Lipids • Nucleic Acids
Proteins: • Account for more than 50% of the dry mass of most cells, and are instrumental in almost everything organisms do. We will cover these in great detail.
Lipids: • Monomer- monoglycerides and fatty acids • Polymer-Triglycerides: Glycerol, and 3 Fatty acid chains • Phospholipids • Jobs in the body: • Long-term energy storage • Makes up cell membranes • Sending messages to distant cells (hormones) • Insulation and protection of us and our cells!
Carbohydrates: (sugar!) • Main source of energy for life on earth • Monomer-"Simple sugars" monosaccharides (glucose, ribose) • Jobs in the body: • Immediate energy • Labeling a cell • Cell walls in plants
Nucleic Acids: • Monomer: Nucleotide • Composed of a nitrogenous base, a sugar, and a phosphate group. • Combine to form DNA • Polymer, DNA, RNA
Food Examples • Uhhhhhhhhhhhhhhhhhhhh……… • Stomach Contents Lab next class!!!