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(a) Q-PCR GSC standard curve

(a) Q-PCR GSC standard curve. Ct = -5.136 x log(C’) + 31.083 (R 2 = 0.9837) * C’ = 10 ^ ((Ct-31.083)/(-5.136)). Ct value. log(C’) C’=copy number per cell in sample homogenate. (b) GeneChip GSC standard curve. log(S) S=GeneChip signal intensity. log(S) = 0.9846 x log(C’) + 1.2353

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(a) Q-PCR GSC standard curve

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  1. (a) Q-PCR GSC standard curve Ct = -5.136 x log(C’) + 31.083 (R2 = 0.9837) * C’ = 10 ^ ((Ct-31.083)/(-5.136)) Ct value log(C’) C’=copy number per cell in sample homogenate (b) GeneChip GSC standard curve log(S) S=GeneChip signal intensity log(S) = 0.9846 x log(C’) + 1.2353 (R2 = 0.9785) * C’ = 10 ^ ((log(S) – 1.2353)/0.9846) log(C’) C’=copy number per cell in sample homogenate

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