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Analysis of miRNA Interactions and Gene Expression in Takeda et al. Supplemental Figures

This document presents an analysis of figures from Takeda et al., focusing on Supplemental Figures 1, 2, and 3. Figure 1 explores sequence height variations and the comparison of gDNA mixes using different experimental setups. Figure 2 examines the effects of fixation and sonication on miRNA interactions with hAGOs. Lastly, Figure 3 displays quantitative log2 transformations of input versus immunoprecipitation (IP) results, providing insights into the miRNA and gDNA dynamics. The findings contribute to understanding regulatory mechanisms at the molecular level.

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Analysis of miRNA Interactions and Gene Expression in Takeda et al. Supplemental Figures

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  1. Takeda et al., Supplemental Figure 1 (A) (B) A / (A+G) Sequence Height Sequence Height A / (A+G) pRL-TK-4xA/4xG mix A/(A+G) pRL-TK-3’A/3’G mix A/(A+G) (C) (D) GeneScan Area A / (A+G) Sequence Height A / (A+G) gDNA mix AA/(AA+GG) gDNA mix AA/(AA+GG)

  2. Takeda et al., Supplemental Figure 2 (A) No Fixation With Fixation Input Sup IP Input Sup IP Sonication L L H H L L H H L L H H L L H H L H H L L H H miRNA 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1M 1 1M 1 1M 1 1M hAGOs * h l (B) No Fixation With Fixation Sonication L L H H L L H H miRNA 1 1M 1 1M 1 1M 1 1M + - + - + - + - + - + - + - + - RT Input IP Log2 [ (A/GIP) / (A/Ginp) ]

  3. Takeda et al., Supplemental Figure 3 Input IP Log2 [ (A/G) / (A/G gDNA) ]

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