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5’ dTP-PCR

5’ dTP-PCR. 3’ dTP-PCR. 85 °C. -dF/dT. 90 °C. -dF/dT. + GM06891 (PM male). Normal male blood spot. + GM07862 (FM male). + NA20244 (NL male, 41 rpts). A. + NA20244 (NL male, 41 rpts). + GM06891 (PM male). + GM07862 (FM male). Normal male blood spot. NA20244 (NL male, 41 rpts).

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5’ dTP-PCR

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  1. 5’ dTP-PCR 3’ dTP-PCR 85 °C -dF/dT 90 °C -dF/dT + GM06891 (PM male) Normal male blood spot + GM07862 (FM male) + NA20244 (NL male, 41 rpts) A + NA20244 (NL male, 41 rpts) + GM06891 (PM male) + GM07862 (FM male) Normal male blood spot NA20244 (NL male, 41 rpts) GM04738 (NL male, 30 rpts) GM06891 (PM male) NA20244 (NL male, 41 rpts) GM07862 (FM male) B GM06891 (PM male) GM07862 (FM male) GM04738 (NL male, 30 rpts) 70 72 74 76 78 80 82 84 86 88 90 92 94 70 72 74 76 78 80 82 84 86 88 90 92 94 Temperature (°C) Temperature (°C) Supplemental Figure 5: (A) Melt peaks of blood spots from a normal male mixed with genomic DNA from NA20244 (41 repeat male), GM06891 (premutation male) and GM07862 (full mutation male); (B) Melt peaks from male genomic DNA samples GM04738 (30 repeats), NA20244 (41 repeats), GM06891 (premutation) and GM07862 (full mutation).

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