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Protein Expression Study: Influenza Virus NS1 Mutations Impact Localization and Function

This study investigates the impact of E186A-W187S mutation on Influenza A NS1, providing insights into nucleus-specific functions. Immunoblotting and immunofluorescence data are presented.

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Protein Expression Study: Influenza Virus NS1 Mutations Impact Localization and Function

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  1. Protein Expression kindly provided by Dr. Shin-Ru Shih A/WSN/33/(H1N1): InfA_WT E186A-W187S mutation by single primer Primer sequence: GAATGG ggtcctcatcggaggacttGCTAGCaataataacacagttcgagtctctga -InfA_E186AW-187S -InfA_WT pCR3.1 InfA_WT InfA_E186A-W187S 43 34 Fig. 1b: -Flag immunoblotting, molecular weight for influenza A NS1: 30 kDa Fig. 1a: -Flag IFA, nucleus predominant

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