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CK8

N-Cadherin. Fibronectin. Twist. CK8. CK14. CK17. CK18. CK19. CK5. CK6. Vimentin. CK20. EpCAM. Muc1. EGFR. panCTC. panCK. CK19. Supplementary Figure 1. B. A. C. D.

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CK8

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  1. N-Cadherin Fibronectin Twist CK8 CK14 CK17 CK18 CK19 CK5 CK6 Vimentin CK20 EpCAM Muc1 EGFR panCTC panCK CK19 Supplementary Figure 1. B A C D Supplemental Figure 1. Marker development for CTC and blood cells.(A) MDA-MB-468 cells were spiked into PBMC and hybridized with probes to detect epithelial cell marker mRNAs including CK8, CK14, CK17, CK18, CK19, CK20, EpCAM, Muc1, and EGFR. (B) MRC-5 cells were spiked into PBMC and hybridized with probes to detect EMT marker mRNAs, including Twist, N-Cadherin, and Fibronectin. (C) PBMCs showing epithelial or EMT marker mRNAs, including CK5, CK6, and Vimentin. (D) MDA-MB-468 cells were spiked into PBMC and hybridized with a probes to detect CK19 mRNA , CK8, 18, and 19 mRNAs (panCK), or CK8, CK14, CK17, CK18, CK19, CK20, EpCAM, Muc1, EGFR, Twist, N-Cadherin, and Fibronectin mRNAs (panCTC). Cells were counterstained with DAPI (blue).

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