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A cancer drug exploration

Secrets of the Rainforest. A cancer drug exploration. The Mysterious Green Florescent Leaves. Hi, I am Tisha. While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal green leaves from Ramon

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A cancer drug exploration

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  1. Secrets of the Rainforest A cancer drug exploration

  2. The Mysterious Green Florescent Leaves Hi, I am Tisha • While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal green leaves from Ramon • You bring back to Biotex, a biotech company working on a cure for cancer

  3. Green Florescent Protein • Biotex scientists determine that the leaves contain a florescent green protein • DNA from the leaves is inserted into bacterial cells

  4. Cloning the Gene from Mysterious Leaves

  5. More on Cloning the Gene…

  6. Purifying the GFP • Biotex technicians need to separate the GFP from other bacterial proteins • Column Chromatography separates contaminating proteins from GFP • The result is a purified GFP that can be tested for its ability to cure stomach cancer

  7. Testing and Placebos • GFP will need to be tested on rats before it is allowed for a human trial • The US Food and Drug Administration will examine Biotex’s experiments to determine if the protein is safe and cures stomach cancer without negative side effects

  8. Ethics and Economics • Once GFP is approved by the FDA, you will develop GFP as a commercial product • You will need to be aware of the following viewpoints: FDA, social conscience, patient advocacy groups, and the employees of Biotex • You must resolve the dilemmas in order to successfully market GFP

  9. Final Presentations • Turn in all lab work with analysis questions • Video Presentation of your marketing of GFP • Address issues of all dilemmas 1-8

  10. Background Information- Lesson 1 • How to use Pipettes • Streaking of agar plates to Produce Single Colonies (Sterile Technique)

  11. Background Continued…Lessons 2 & 3 • Process of Transformation (GMOs or genetic recombinants) • Jellyfish , GFP and pGLO and Fluorescence • Isolating Clones to Propagate in Liquid Culture Media

  12. Background Continued…Lesson 4 • Concentration of Bacteria from Liquid Culture Media

  13. Background Continued…Lesson 5 • Prepare a Bacterial Lysate for Protein Chromatography

  14. Background Continued…Lesson 6 • Protein Chromatography-Hydrophobic Interaction Chromatography (HIC) • GFP is hydrophobic and will stick to hydrophobic beads in salt water buffer • When salt is removed, GFP will then be collected • 4 buffers: • 1) Equilibration buffer (salt buffer) • 2) Binding Buffer (high salt) • Wash buffer (medium salt) • Elution buffer (low salt)

  15. Background Continued…Lessons 7-10 • Lesson 7- testing and placebos • Lesson 8- Advocacy Groups and Viewpoints • Lesson 9-Ethics and Economics • Lesson 10- Preparing and Giving Presentations

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