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Arbovirus Surveillance: Laboratory Tests

Arbovirus Surveillance: Laboratory Tests. Utah West Nile Virus Conference February 9, 2004 Dr. June Inez Pounder Utah Department of Health Laboratory. Surveillance –Lab Tests 2003. Human- WNV IgM ELISA 36 Utah residents tested 1 + Uintah County 9/18 4 + Travel associated

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Arbovirus Surveillance: Laboratory Tests

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  1. Arbovirus Surveillance:Laboratory Tests Utah West Nile Virus Conference February 9, 2004 Dr. June Inez Pounder Utah Department of Health Laboratory

  2. Surveillance –Lab Tests 2003 • Human- WNV IgM ELISA • 36 Utah residents tested • 1 + Uintah County 9/18 • 4 + Travel associated • More tested for test validation

  3. Surveillance-Lab Tests 2003 • Mosquito-Real-time PCR • 1945 pools total • 100% Culex tarsalis, Culex pipiens • 2 positive C. tarsalis • Uintah, Utah County 8/11 • Culex erythorthorax in Moab • activity no target species

  4. Surveillance-Lab Tests 2003 • Avian-swabs and tissue by RT-PCR • 162 samples total • 126 oral cavity swabs UDOH • 11 tissue samples UVDL • 24 live swabs Tracy Aviary-Cornell University • 2 + Grand County • Crow 9/10 • Magpie 10/9

  5. Surveillance-Lab Tests 2003 • Sentinel Chickens-IgM ELISA • 280 birds 16 weekly bleeds UVDL • 9 seroconversions WNV • Carbon County 2 birds 8/4 2 birds 9/2 • Emery County 4 birds 8/18 • Duchesne County 1 bird 9/8

  6. Surveillance- Lab Tests 2003 • Equine-WNV IgM ELISA • 99 total UVDL • 35 + 6 counties • Duchesne 3 Uintah 16 • Emery 8 Wayne 2 • Millard 4 Sanpete 2

  7. Surveillance-Lab Tests 2004 • Human • WNV IgM ELISA-meningoencephalitis • WNV IgM ELISA-confirm Commercial lab (+) • SLE IgM ELISA • WNV PCR immunocompromised • Mosquito • Culex tarsalis, C. pipiens all tested • C. erythrothorax-activity in area, special

  8. Surveillance-Lab Tests 2004 • Birds • Oral swabs • Activity established/cease testing • Sentinel Chicken Serology • Increased # flocks • Replace seroconverted birds

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