T and B Cell Costimulation

T and B Cell Costimulation Robert H. Carter, MD rcarter@uab.edu Div. Rheumatology, Dept. Medicine Dept. Microbiology Program in Cellular and Molecular Biology Program in Immunology University of Alabama at Birmingham

Germinal Center, WT CD19, SRBC Day 7 Red pulp FDC Germinal Center Marginal zone Marginal Sinus Endothelial cell Central arteriole PNA Alexa 488 GreenCD35 Alexa 350 Blue Ki-67 Alexa 555 RedIgD Bio SA-Cy5 gold

Memory B cell After immunization Plasma Cell PC Germinal Center T B B B T B Cell Structure of a Follicle hypermutation affinity maturation class switch

Ag B cell binds antigen Ag internalization and processing Cell death Antigen Binding and Processing CD40 mIgM MHC Class II B cell

CD28 Resting T Cell T Cell Receptor T cell

CD40 naive anergy T cell CD28 Partial T-B Collaboration-Anergy

activated antigen presenting cell- B cell or APC other sig. CD40L Priming of T Cell-CD40L CD28 T cell

viability Germinal Center class switch memory CD40L primed T cell IL-2 proliferation CD28 CD80 T-B Collaboration-CD80 CD40

primed T cell CTLA4 CD28 primed T cell inhibition Primed B Cell CTLA4 CD80 T-B Collaboration-CTLA4 CD28 Primed B Cell CD80

Blocking Agents anti-CD40L CD40L B cell T cell CD28 CD80 CTLA4Ig Collaboration blocked ? Anergy

T and B Cell Costimulation