No. 46 Phlorotannin, an extract of Ecklonia cava, and its anti-inflammatory effect on chronic nonbacterial prostatitis in a rat model Kevin Lah1, Yun Seok Jung2, Mun Su Chung2, Yong Hyun Cho2, Chang Hee Han2 1 Royal Brisbane and Women’s Hospital, Queensland, Australia 2 Department of Urology, College of Medicine, The Catholic University of Korea Posters Proudly Supported by: Results Histology: There were no signs of chronic inflammation in control group (Fig. 1) and E. cava-fed group. HIP, E. cava-treated HIP, and NSAID-treated HIP groups showed evidences of chronic inflammation (Figs. 2-4). E. cava-treated HIP and NSAID-treated HIP groups had significantly less inflammation compared with HIP group (P<0.05). There were no statistically significant differences between E. cava-treated HIP group and NSAID-treated HIP group (P > 0.05). (Fig. 5). Oxidative stress marker: The MDA levels were significantly increased in HIP, E. cava-treated HIP and NSAID-treated HIP groups compared with control and E. cava-fed groups (P < 0.05). The MDA levels were significantly less in E. cava-treated HIP group compared with HIP group (P < 0.05). The MDA value was lower in NSAID-treated HIP group than in HIP group, however this was not statistically significant (P > 0.05). (Fig. 6). Introduction Chronic nonbacterial prostatitis (CNP) and chronic pelvic pain syndrome account for 90-95% of all prostatitis. Little is known about its pathophysiology and various treatments are utilised. Eckloniacava is a seaweed in brown algae family, and its extract, phlorotannin has been shown to have anti-oxidative and anti-inflammatory properties. Aim To investigate the anti-inflammatory and anti-oxidative effects of phlorotannin in Ecklonia cava on chronic nonbacterial prostatitis using the hormone-induced prostatitis rat model. Fig. 1. Control prostate (x200). Fig. 2.Hormone-induced prostatitis (x200). Methods Forty 10 week-old male white Wistar rats were divided equally into 5 groups: 1) Control, 2) E. cava-fed, 3) Hormone-induced prostatitis (HIP), 4) E. cava-treated HIP, and 5) NSAID-treated HIP. Prostate histological analysis was performed by 2 blinded pathologists reporting on three criteria: infiltration of chronic inflammatory cells, the change of the acinar, and interstitial fibrosis. Malondialdehyde(MDA) oxidative stress marker was analysed in each prostate tissues, which was used inversely as a surrogate marker of anti-oxidative properties. All data were analysed and expressed as mean ± standard deviation. Groups were compared using ANOVA followed by Tukey’s test for multiple comparisons. The level of significance was p <0.05. Fig. 5. Histology severity scores. *P<0.05 compared with HIP group. Fig. 3. E. cava-treated HIP (x200). Fig. 4. NSAID-treated HIP (x200). Conclusions This study demonstrates that phlorotannin has anti-inflammatory and anti-oxidative properties, evidenced in reduced histological change and decreased oxidative stress in a CNP animal model. We expect phlorotannin to play an important role in prevention and as an adjuvant therapy for the management of CNP. Fig.6. MDA values in each group. *P<0.05 compared with control group; +P<0.05 compared with HIP group. Acknowledgements None References Naslund MJ, Strandberg JD, Coffey DS. The role of androgens and estrogens in the pathogenesis of experimental nonbacterial prostatitis. J Urol 1988;140:1049-53.