1 / 1

Bisulfite Conversion and PCR Amplification of DNA Sequences for Methylation Analysis

This document details the bisulfite conversion and PCR amplification of specific DNA sequences for methylation analysis. It includes information about the top and bottom strands of DNA after conversion, along with the necessary PCR primers. The sequences provided are crucial for studying DNA methylation patterns, particularly focusing on CpG dinucleotides. The key highlight is the conversion process that enables the differentiation of methylated and unmethylated cytosines, facilitating epigenetic studies and applications in genomics research.

tracen
Télécharger la présentation

Bisulfite Conversion and PCR Amplification of DNA Sequences for Methylation Analysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. H19 DMR 5’ 3’ GTAGATTTGGTTATAGTTAAATGGATA----ATTTTATAGTTATAAAAGTAGAGGTTGG CATCTAAACCAATATCAATTTACCTAT----TAAAATATCAATATTTTCATCTCCAACC 3’ 5’ PCR 5’ 3’ GTAGATTTGGTTATAGTTAAATGGATA----ATTTTATAGTTATAAAAGTAGAGGTTGG Converted top strand Bisulfite 5’ 3’ GCAGATTTGGCTATAGCTAAATGGACA----ACCCCATAGCCATAAAAGCAGAGGCTGG CGTCTAAACCGATATCGATTTACCTGT----TGGGGTATCGGTATTTTCGTCTCCGACC 3’ 5’ (Beware of CpG dinucleotides in primer sequence) Bisulfite TGTTTAAATTGATATTGATTTATTTGT----TGGGGTATTGGTATTTTTGTTTTTGATT 3’ 5’ Converted bottom strand PCR 5’ 3’ ACAAATTTAACTATAACTAAATAAACA----ACCCCATAACCATAAAAACAAAAACTAA TGTTTAAATTGATATTGATTTATTTGT----TGGGGTATTGGTATTTTTGTTTTTGATT 3’ 5’

More Related