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Lateral Flow Immunochromatographic Assay - In vitro diagnostics using colloidal gold

Lateral Flow Immunochromatographic Assay - In vitro diagnostics using colloidal gold. Outline. Introduction Technology Principle – How it works Applications . Introduction. Lateral flow test Paper strip In vitro diagnostics Simple system Fast system, results in 10-20 min

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Lateral Flow Immunochromatographic Assay - In vitro diagnostics using colloidal gold

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  1. Lateral Flow Immunochromatographic Assay- In vitro diagnostics using colloidal gold

  2. Outline • Introduction • Technology • Principle – How it works • Applications

  3. Introduction Lateral flow test • Paper strip • In vitro diagnostics • Simple system • Fast system, results in 10-20 min • Detects presence/absence of target analyte • Limitless potential applications

  4. Technology • Sample pad • Conjugate pad • Membrane • Test line and control line (immobilized antibodies) • Absorption pad

  5. Technology • Colloidal gold antibody conjugate • Colloidal gold (10-50 nm in diameter) • Antibodies binds to target analyte • Accumulation of conjugates gives a red color visible for the naked eye in the given area

  6. Principle – How it works Sample pad Conjugate pad Absorption pad Test zone Control zone Anti-analyte Anti-antibody Membrane Anti-analyte CGC

  7. Principle – How it works • Diagnostic interpretation Control line Test line Positive test Negative test Invalid test

  8. Applications • Limitless • Home diagnostics (e.g. pregnancy test) • Point of care diagnostics (e.g. HIV, malaria) • Laboratory

  9. Questions?

  10. Reference list • NGOM, B., GUO, Y., WANG, X. and BI, D., 2010. Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: A review. Analytical and Bioanalytical Chemistry, 397(3), pp. 1113-1135 • POSTHUMA-TRUMPIE, G.A., KORF, J. and VAN AMERONGEN, A., 2009. Lateral flow (immuno)assay: Its strengths, weaknesses, opportunities and threats. A literature survey. Analytical and Bioanalytical Chemistry, 393(2), pp. 569-582 • SHAH, P., ZHU, X. and LI, C.-., 2013. Development of paper-based analytical kit for point-of-care testing. Expert Review of Molecular Diagnostics, 13(1), pp. 83-91 • VERHEIJEN, R., STOUTEN, P., CAZEMIER, G. and HAASNOOT. W., 1998. Development of a one step strip test for the detection of sulfadimidine residues. Analyst, 123, pp. 2437-2441

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