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Distribution of γH2AX and HIRA in Young and Old NHOST and AG11016 Fibroblasts

This study explores the distribution of γH2AX and HIRA proteins in young and old NHOST and AG11016 skin fibroblasts, focusing on early and late passage effects. Utilizing techniques such as immunofluorescence and BrdU incorporation assays, we examine the cellular responses to stressors and senescence. The findings reveal significant differences in protein expression and cellular behavior influenced by age and passage conditions, contributing to our understanding of cellular aging and its implications for tissue health.

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Distribution of γH2AX and HIRA in Young and Old NHOST and AG11016 Fibroblasts

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  1. AG11016 TIF assay Young Old 53 Bp1 γH2AX

  2. NHOST TIF assay Young Old 53 Bp1 γH2AX

  3. HIRA Young Old AG11016 NHOST

  4. NHOST Early Passage Late Passage

  5. NHOST 40x Early passage/Toxic Early passage/Quiescent

  6. Ag11016 10x 40x 40x 10x Late Passage Early Passage

  7. Ag11016 Toxic Senescent

  8. HIRA distribution in AG11016 skin fibroblasts

  9. HIRA distribution in NHOST osteoblasts

  10. Passage and CPDL

  11. Passage and dPDL

  12. BrdU Incorporation

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