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This study explores the distribution of γH2AX and HIRA proteins in young and old NHOST and AG11016 skin fibroblasts, focusing on early and late passage effects. Utilizing techniques such as immunofluorescence and BrdU incorporation assays, we examine the cellular responses to stressors and senescence. The findings reveal significant differences in protein expression and cellular behavior influenced by age and passage conditions, contributing to our understanding of cellular aging and its implications for tissue health.
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AG11016 TIF assay Young Old 53 Bp1 γH2AX
NHOST TIF assay Young Old 53 Bp1 γH2AX
HIRA Young Old AG11016 NHOST
NHOST Early Passage Late Passage
NHOST 40x Early passage/Toxic Early passage/Quiescent
Ag11016 10x 40x 40x 10x Late Passage Early Passage
Ag11016 Toxic Senescent