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Facility Status and Highlights of the AMRIS MRI/S Program

NHMFL development cores in HTS NMR probes, novel contrast agents for use at high fields, and microimaging are now leveraged by NIH funding R01 to fund 1.5-mm HTS 13 C-optimized probe construction began in fall 2009

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Facility Status and Highlights of the AMRIS MRI/S Program

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  1. NHMFL development cores in HTS NMR probes, novel contrast agents for use at high fields, and microimaging are now leveraged by NIH funding • R01 to fund 1.5-mm HTS 13C-optimized probe construction began in fall 2009 • R01 application for development of MR Microscopy at the cellular level is at 2nd percentile • U54 to study failed regeneration in the muscular dystrophies using novel contrast approaches is one of three anticipated funded centers in the country. • Three of seven instruments in the facility upgraded/will receive upgrade in 2010 and early 2011. This is a $1.4M investment in the facility with $0.3M from NHMFL funding. • New partnership with UF Chemistry and CTSI to develop center for metabolomics integrating 1.5-mm HTS 13C-optimized probe, developed with Agilent, on a new 600 MHz instrument. This instrument, a $800k investment with $100k from the NHMFL, will be part of the NHMFL external user program. • Hired a PhD level Staff Scientist for Animal Imaging to support UF and NHMFL external user programs. • Partnered with high B/T facility on successful ARI-R2 proposal to revitalize the UF helium liquefaction and recovery system. Facility Status and Highlights of the AMRIS MRI/S Program as of summer 2010, mid-point in current award period

  2. Science Highlights of the AMRIS MRI/S Program • First MRI microscopy of mammalian neurons and subcellular structures • Two cover articles in NeuroImage • Imaging with direct histological characterization of nucleoli in frog ova • Development of stronger gradients and perfusion probe with Bruker • High resolution brain white matter fiber structure • Fiber tract connections between major anatomical regions • Transport models for convection-enhanced CNS delivery • Natural product identification • ~1 mL of a “defensive ooze” from a leaf insect • We found 3 related pyrazines (smells like chocolate!) • Required the 1-mm HTS probe (right before it broke…) • Gene reporter for detection of cellular LacZ expression by MRI Pre Sgal Post Sgal -LacZ as of summer 2010, mid-point in current award period

  3. Development of NMR and MRI/S program at 30T and beyond • Microimaging program (1-2 mm3 FOV at micron resolution) • Measuring and matching of magnetic susceptibility • Development of contrast agents • NMR of biomolecular systems using microvolumes • Accelerated acquisition techniques • Managing and measuring anisotropic interactions • Transformational research • Signal detection in an extreme environment • Flow and diffusion over multiple length and timescales in deep structures • Bridging atomic and anatomical scales in biology • Systems biology and chemical communication • Renovation/expansion of AMRIS facility space • Development of program in multi-transmit/receive MRI Visionfor the AMRIS MRI/S Program as of summer 2010, mid-point in current award period

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