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Specii din genul Actinobacillus si Haemophilus cu semnificatie patologica pentru porc

Specii din genul Actinobacillus si Haemophilus cu semnificatie patologica pentru porc. Dr. COSTIN STOICA www.tgw1916.net. REGNVM PROKARYOTAE. FAMILIA PASTEURELLACEAE. PASTEURELLA ACTINOBACILLUS HAEMOPHILUS. GENURI:. REGNVM PROKARYOTAE.

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Specii din genul Actinobacillus si Haemophilus cu semnificatie patologica pentru porc

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  1. Specii din genul Actinobacillus si Haemophiluscu semnificatie patologica pentru porc Dr. COSTIN STOICA www.tgw1916.net REGNVM PROKARYOTAE

  2. FAMILIA PASTEURELLACEAE • PASTEURELLA • ACTINOBACILLUS • HAEMOPHILUS GENURI: REGNVM PROKARYOTAE

  3. Dendrograma privind relatiile filogenetice ale unor reprezentanti ai familiei Pasteurellaceae pe baza similaritatii secventei ARNr 16S A. actinomycetemcomitans H. aphrophilus H. segnis H. haemolyticus H. influenzae H. aegyptius A. equuli A. suis A. ureae A. pleuropneumoniae A. lignieresii A. minor H. minor group A. porcinus A. indolicus H. parasuis (NCTC 4557) H. parasuis (strain Nagasaki) Pasteurella species A H. taxon C P. gallinarum H. paragallinarum

  4. GENUL ACTINOBACILLUS • A. actinomycetemcomitans • A. lignieresii • A. equuli • A. seminis • A. capsulatus • A. pleuropneumoniae • A. minor • A. porcinus • A. indolicus • A. suis • A. ureae • A. rossii • A. muris • A. hominis ( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996) REGNVM PROKARYOTAE

  5. GENUL ACTINOBACILLUS • A. actinomycetemcomitans • A. lignieresii • A. equuli • A. seminis • A. capsulatus • A. pleuropneumoniae • A. minor • A. porcinus • A. indolicus • A. suis • A. ureae • A. rossii • A. muris • A. hominis ( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996)

  6. Specii din genul Actinobacillus cu importanta pentru porc • A. pleuropneumoniae • A. minor • A. porcinus • A. indolicus • A. suis REGNVM PROKARYOTAE

  7. Toxinele sintetizate de fiecare serotip de A. pleuropneumoniae REGNVM PROKARYOTAE

  8. Actinobacillus suis – coloratie Gram

  9. Caractere biochimice diferentiale intre reprezentanti ai genului Actinobacillus + : >90% tulpini pozitive, - : > 90% tulpini negative, d : 11- 89% tulpini pozitive (Moler et al. , 1996)

  10. GENUL HAEMOPHILUS • H. parasuis • H. taxon C • H. taxon D • H. taxon E • H. taxon F • H. ‘minor group’ • H. aegyptius • H. influenzae • H. aphrophilus • H. haemolyticus • H. segnis • H. paragallinarum REGNVM PROKARYOTAE

  11. GENUL HAEMOPHILUS • H. parasuis • H. taxon C • H. taxon D • H. taxon E • H. taxon F • H. ‘minor group’ • H. aegyptius • H. influenzae • H. aphrophilus • H. haemolyticus • H. segnis • H. paragallinarum

  12. Specii din genul Haemophilus cu importanta pentru porc • H. parasuis • H. taxon C • H. taxon D • H. taxon E • H. taxon D • H. minor group REGNVM PROKARYOTAE

  13. Haemophilus parasuis – coloratie Gram

  14. Caractere biochimice H.minor group H. parasuis Taxon C Taxon D Taxon E Taxon F Necesar factor V 100 100 100 100 60 100 Necesar factor X 0 0 0 0 0 0 Indol 0 0 0 0 0 100 Ureaza 100 0 0 0 0 0 Oxidaza 13 100 100 0 0 100  galactozidaza 100 100 0 100 80 100  fucosidaza 0 96 0 68 80 46  glucozidaza 70 4 100 64 20 89 Neuraminidaza 100 100 100 91 60 100 Red. nitratilor 100 100 100 96 30 100 Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilus

  15. Caractere biochimice H.minor group H. parasuis Taxon C Taxon D Taxon E Taxon F Arabinoza 0 0 100 86 0 0 Lactoza 91 0 0 96 0 32 Maltoza 100 100 100 100 20 100 Melibioza 78 0 0 96 0 18 Sucroza 100 100 100 100 10 100 Trehaloza 78 0 0 55 0 79 Rafinoza 100 0 100 100 0 93 Manitol 0 0 0 100 0 0 Sorbitol 0 0 0 100 0 0 Inozitol 0 4 100 77 0 0 Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilus (continuare)

  16. BIBLIOGRAFIE: Andrew N. Rycroft, Lisa H. Garside, " Actinobacillus Species and their Role in Animal Disease", The Veterinary Journal , 2000, 159, 18-36 Cristian Moller et al., ‘V Factor Dependent Members of the Family Pasteurellaceae in the Porcine Upper Respiratory Tract’, Journal of Clinical Microbiology, Dec 1990, p. 2711-2716 Durda Slavic et al. " Prevalence of O1/K1 and O2/K3 Reactive A. suis in Healthy and Diseased Swine", Journal of Clinical Microbiology, Oct 2000, p. 3759-3762 Durda Slavic et al. "Comparative pathogenicity of different Actinobacillus suis O/K serotypes", The Canadian Journal of Veterinary Research, 81-86 J. Van Ostaaijen et al., " A. suis Strains Isolated from Healthy and Diseased Swine Are Clonal and Carry apxICABDvar suis and apxIICAvar suis Toxin Genes", Journal of Clinical Bacteriology John G. Holt et al., “Bergey’s Manual of Determinative Bacteriology 9th Edition, 1994 Moller K. et al., Oct. 1996, "A. minor sp. nov., A. porcinus sp. nov., and A. indolicus sp. nov., Three New V Factor-Dependent Species from the Respiratory Tract of Pigs", International Journal os Systematic Bacteriology , Oct. 1996, 1131-1137   Radu Moga M@nzat et al., “Boli infec\ioase ale animalelor – bacterioze”, p. 157-188, Ed. Brumar 2001 Vicki J. Rapp-Gabrielson et al., "Prevalence of Haemophilus parasuis serovars among isolates from swine", Am. J. Vet. Res. Vol 53, No 5, 1992

  17. THE END REGNVM PROKARYOTAE

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