1 / 10

Polymerase Chain Reaction

Polymerase Chain Reaction. DNA Structure: Two strands in helix. Adenine ( A ) binds to Thymine ( T ), with two hydrogen bonds. Guanine ( G ) binds to Cytosine ( C ), with three hydrogen bonds. DNA. Helix is double-stranded & “antiparallel” Primary strand (sense)

verna
Télécharger la présentation

Polymerase Chain Reaction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Polymerase Chain Reaction

  2. DNA Structure:Two strands in helix Adenine ( A ) binds to Thymine ( T ), with two hydrogen bonds. Guanine ( G ) binds to Cytosine ( C ), with three hydrogen bonds.

  3. DNA Helix is double-stranded & “antiparallel” • Primary strand (sense) 5’- CCGAATGGGATGC -3’ 3’- GGCTTACCCTACG -5’ • Complementary strand (antisense)

  4. PCR- Recap • Using a specific cocktail and varying temperatures/times… • TARGET & AMPLIFY desired DNA segment

  5. PCR Quiz • What are the 3 stages? • Temperatures? • What’s Happening? • In the end: How do you know if it worked?

  6. The temp cycle of PCR

  7. How Does a Gel Work? • Check a PCR sample by gel electrophoresis. +

  8. How Do you make a gel? 1% gel: Mix 0.4 grams agarose with 40ml 1X TBE buffer and heat (add 1 ul 10mg/ml ETBR stain right before pouring into tray)

  9. What’s the story, the hypothesis and was it supported?

More Related