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Schematic Representation of the pBI9526Ω Vector and Its Components

This document provides a detailed schematic representation of the 9526Ω vector, a derivative of the pBI221 vector commonly used in plant transformation. Key features include the P35S promoter derived from the cauliflower mosaic virus, the Ω omega sequence for stability, and the GUS gene (β-glucuronidase) for expression analysis. The Tnos region, originating from the nopaline synthase gene, is also included to enhance transcription termination. This vector's design and components allow for effective genetic studies in plant systems.

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Schematic Representation of the pBI9526Ω Vector and Its Components

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  1. XbaI SpeI BaｍHI SmaI pBI9526Ω Ω P35S Tnos ＧＵＳ ApaI XhoI EcoRI KpnI BstxI ApaI XhoI EcoRI KpnI BstxI Bgl II HindIII PstI SacI SalI NotI Supplementary Fig. S1 Schematic representation of the 9526Ω vector 9526Ω is a derivative of pBI221 vector. P35S, cauliflower mosaic virus 35S promoter; Ω, omega sequence; GUS, β-glucronidase gene; Tnos, 3’ region of nos.

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