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PCR Amplification and Digestion of SRK Class I Gene in Rapid Cycle B. oleracea

PCR amplification and digestion of SRK class I gene in B. oleracea 'TO1000DH3' using KD5 and KD8 primers. Visualization of alignment of kinase domains of TO1000DH3 and BoS-7.

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PCR Amplification and Digestion of SRK Class I Gene in Rapid Cycle B. oleracea

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  1. HinfI+EcoRII MspI HinfI AluI AfaI M bp 500 200 100 TGGAAGGACAGATTCGCCATTACAAATGGTGTTGCTCGAGGGCTTTTATATCTTCATCAAGACTCACGGTTTAGGATAATCCACAGGGATTTGAAACCAGGTAACATTTTGCTTGATAAATATATGATCCCAAAGATCTCGGATTTTGGGATGGCCAGAATCTTTGCAAGGGACGAAACTCAAGCTAGGACAGACAATGCGGTCGGAACTTA‍Gtaagcaagtctttgaagatattttcttacccataactctatagaactcatgacctctaagttcattttttttgctactcag‍CGGGTACATGTCTCCGGAGTACGCAATGGATGGGGTAATCTCGGAAAAAACAGATGTTTTCAGTTTTGGAGTCATAGTTCTTGAAATTGTTAGTGGAAAAAGGAATAGAGGATTCTACCAGGTGAACCCTGAAAACAATCTTCTAAGCTAT‍Gtaagttaaagaaccaataatattctatctactctcgaaattgctaaaacacttttttttaacaacgctaaaacaattgaaatgcttttatttttataaacag‍GCATGGAGTCATTGGGCGGAGGGAAGAGCGCTAGAAATCGTAGATCCAGTCATCGTAGATCCATTGGCATCTCTGCCATCAACATTTCAACCAAAAGAAGTCCTAAAATGCATACAAATTGGTCTATTGTGTATTCAAGAACGTGCGGAGCACAGACCAACGATGTCGTCGGTGGTTTGGATGCTTGGAAGTGAAGCAACAGAGATTCCTCAGCCTAAACCGCCAGTTTATTGTCTCATAGCAAGTTATTATGCAAATAATCATTCCTCAAGTAGGCAATTCGACGACGATGAATCCTGGACGGTGAACAAGTACACCTGCTCAGTCATCGATGCCCGGTAA‍TATGAAAGC Supplementary Fig. 1 PCR amplification of SRK class I gene (899 bp) of rapid cycle B. oleracea ‘ TO1000DH3’ using KD5 and KD8 primers and subsequent partial digestion with restriction enzymes. The gene sequence is mentioned below with primers and restriction sites.

  2. TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 TO1000DH3 BoS-7 Supplementary Fig. 2 Alignment of nucleotide sequences of the kinase domain of TO1000DH3 and BoS-7. Numbers on the right margin indicate the positions of base pairs. The identical amino acids are marked in dark background, and dissimilar in light

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