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RB GUS P Ubi TEVL Xa3 NOS Hpt LB

BamH I. BamH I. A. Clone MKbF in vector pU1301. B. BamH I. BamH I. HindIII. -691. +37. RB P WRKY13 Xa3 NOS Hpt LB. Clone 12IMKb in vector pI1381. Dra I. Dra I. C. Clone MKb in vector pCAMBIA1301.

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RB GUS P Ubi TEVL Xa3 NOS Hpt LB

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  1. BamHI BamHI A Clone MKbF in vector pU1301 B BamHI BamHI HindIII -691 +37 RB PWRKY13 Xa3 NOS Hpt LB Clone 12IMKb in vector pI1381 DraI DraI C Clone MKb in vector pCAMBIA1301 RB GUSPUbi TEVL Xa3 NOS Hpt LB RB GUSPXa3 Xa3 NOS Hpt LB Supplemental Figure 1.Schematic diagrams of the transformation constructs. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; PUbi, maize ubiquitin gene promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. (A) overexpression construct of Xa3, MKbF, containing the complete genomic sequence of Xa3 coding region. (B) Pathogen-induced construct of Xa3, 12IMKb, containing the complete genomic sequence of Xa3 coding region. PWRKY13 is OsWRKY13 gene promoter locating at -691 to +37 of OsWRKY13 of rice variety Minghui 63. (C) Construct containing a 7.5-kb DNA fragment harboring Xa3 and its native promoter (PXa3) from Minghui 63.

  2. 10 Zhonghua 11 background Minghua 63 9 8 MKbZH2 (PXa3:Xa3) Zhonghua 11 (wild type) 7 MKbFZH1 (PUbi:Xa3) 6 5 4 3 2 Bacteria [log (cfu/leaf)] Mudanjiang 8 background 10 Minghua 63 9 Mudanjiang 8 (wild type) 8 Rb17-2 (PXa3:Xa3) 7 MKbFMDJ2 (PUbi:Xa3) 6 5 4 3 0 6 12 2 Days after inoculation Supplemental Figure 2.Growth of Xoo strain PXO61 in the leaves of rice plants at four-leaf stage. Indica rice variety Minghua 63 is the donor of Xa3. Japonica rice varieties Zhonghua 11 and Mudanjiang 8 are wild type plants. MKbZH2, MKbFZH1 and MKbFMDJ2 are resistant transgenic plants of T1 generation and Rb17-2 is homogeneous transgenic line. The bacterial population was determined from three leave at each time point by counting colony-forming units (cfu).

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