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Biological Agent Identification Technologies

Biological Agent Identification Technologies. Dr. Mildred Donlon. Biological Role of Channels. Single Channel Currents. Patch Pipette. Bilayer Set Up and Boundary Conditions. Side 2: Insulator. Ion. Ionic Channel. End 1: Electrode. Ion. End 2: Electrode. Ion. Ion. Ion.

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Biological Agent Identification Technologies

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  1. Biological Agent Identification Technologies Dr. Mildred Donlon

  2. Biological Role of Channels Dr. Donlon: Biological Agent Identification Technologies

  3. Single Channel Currents Dr. Donlon: Biological Agent Identification Technologies

  4. Patch Pipette Dr. Donlon: Biological Agent Identification Technologies

  5. Bilayer Set Up and Boundary Conditions Side 2: Insulator Ion Ionic Channel End 1: Electrode Ion End 2: Electrode Ion Ion Ion Ion Ion Ion Side 1: Insulator Membrane Ion Dr. Donlon: Biological Agent Identification Technologies

  6. Ion Channel Protein Ion Ion Ion Ion Ion Ion Channel’s Pore Ion Ion Channel Protein Ions in Channel Protein Dr. Donlon: Biological Agent Identification Technologies

  7. Channels have Inputs and Outputs Channels have Complex Structure that allows Simple Function just like amplifiers! Channels follow simple rules, just like amplifiers Channels as Devices Dr. Donlon: Biological Agent Identification Technologies

  8. Trial and Error or Theory ?? Why Theory? You Choose ! Dr. Donlon: Biological Agent Identification Technologies

  9. Crowded Charge What Theory? Dr. Donlon: Biological Agent Identification Technologies

  10. Selectivity Dr. Donlon: Biological Agent Identification Technologies

  11. It does not matter DFT (Density Functional) Nonner, Gillespie & Eisenberg MC (Monte Carlo) Henderson, et al MSA (Mean Spherical Approximation) Nonner & Eisenberg MCV (Monte Carlo Variational) Hansen et al All give the same results What Version of Theory? Dr. Donlon: Biological Agent Identification Technologies

  12. Make a Calcium Channel ? Dr. Donlon: Biological Agent Identification Technologies

  13. Make a Calcium Channel ?? We are trying !! Dr. Donlon: Biological Agent Identification Technologies

  14. Cl− Channel Dr. Donlon: Biological Agent Identification Technologies

  15. Chloride Channel Selectivity Dr. Donlon: Biological Agent Identification Technologies

  16. Conclusions • Crowded Charge can account for Selectivity • Crowded Charge is important for all Protein Function. Dr. Donlon: Biological Agent Identification Technologies

  17. Future Work Prove it ! Dr. Donlon: Biological Agent Identification Technologies

  18. Many thanks … For a job well done!! To Millie Donlon Dr. Donlon: Biological Agent Identification Technologies

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