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Insulin Receptor Tyrosine Phosphorylation in 3T3-L1 Adipocytes Under Varying Oxygen Levels

This study investigates the effect of oxygen concentration on insulin receptor (IR) tyrosine phosphorylation in 3T3-L1 adipocytes. Cells were incubated for 16 hours at 37°C in environments containing 21%, 7%, or 1% O2. Immunoblots with specific antibodies were utilized to analyze cell lysates. The quantification of IR tyrosine phosphorylation in relation to total insulin receptor levels was assessed across three independent experiments, each conducted in duplicate, providing insight into how oxygen levels influence insulin signaling.

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Insulin Receptor Tyrosine Phosphorylation in 3T3-L1 Adipocytes Under Varying Oxygen Levels

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  1. Online Appendix 1 A. Blot: pTyr IR IR pPKB tubulin + + + Insulin: - - - 21% 7% 1% B. *** % IR phosphorylation + + + Insulin: - - - 21% 7% 1% Legend of Online Appendix 1 A. 3T3-L1 adipocytes were incubated for 16 h at 37°C in 21%, 7% or 1% O2. Cell lysates were analyzed by immunoblots with the indicated antibodies. Representative experiments of three independent experiments done in duplicate are shown. B. Quantification of insulin receptor tyrosine phosphorylation compared to the amount of insulin receptor of three independent experiments done in duplicate is shown.

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