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Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary PowerPoint Presentation
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Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary

Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary

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Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary

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  1. Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary Phone: 36-1-352-2405 Fax: 36-1-413-6944 E-mail: chiroquest@chello.hu web: www.chiroquest.org

  2. Chemically bonded 4-nitrophenyl-urea stationary phases • π-Select • high surface coverage • fully endcapped • CD-Screen • optimized coverage, according to the size of cyclodextrin molecule • free silanols H-acceptor δ+ H-donors hydrophylic hydrophobic

  3. DIMETHYL-PHTALATE click on image for animation

  4. CAFFEINE click on image for animation

  5. PHENOL click on image for animation

  6. Polar analytes π-Select k’=0.54 Conventional phenyl-column k’=0.25 Eluent: MeOH-water 30:70 1.: Phenol 2.: Caffeine

  7. Apolar, aromatic analytes π-Select C: 4.7% Conventional phenyl-column C: 7.0% Eluent: MeOH-water 60:40 1.: Dimethyl-phtalate; 2.: Diethyl-phtalate 3.: Biphenyl 4.: o-Terphenyl

  8. Diastereomer peptides * Tyr-Pro-Phe-Atc-NH2 Eluent: 0.1% TFA in water-0.1% TFA in MeOH 75:25 Atc-NH2: 2-amino-decaline-2-carboxylicacid * : racemic amino acid Basic drugs Eluent: MeOH-0.1% H3PO4 60:40 0.8 ml/min pH: 2.6 1.: N-methyl-ephedrine; 2.: Athenolol 3.: Pindolol; 4.: Sertraline

  9. 1. Benzothiophene 6. Benzo[a]pyrene 3 2 4 2 1 1 7. Benzo[g,h,i]perylene 3 2. Fluorene 7 5 5. Pyrene 6 3. Fluoranthene 4. Dibenzothiophene Separation of Polinuclear Aromatic Hydrocarbons on Pi-Select column methanol-water 85:15 methanol-water 75:25

  10. π-Select Plates: 16685 Asymmetry: 1,189 cis and trans R and S LiChrosphere Si 60 Plates : 5649 Asymmetry : 1,261 Prostaglandine intermediate R * Eluent: n-hexane - TBME 97:3

  11. 1.: dehydroepiandrosterone 2.: testosterone 3.: androstenedione 4.: pregnenolone 5.: dehydroepiandrosterone-sulfate Steroids in blood Juvenile hormone (insecticide) geometrical isomers

  12. CD-SCREEN Inclusion complex with beta-cyclodextrin

  13. click on image for animation

  14. Hydroxypropyl-β-CD the most likely candidate for incorporation into various drug formulations • Nucleosil 120-7 NH2 column, normal phase • solubility problems • β-CD contamination is the last peak, the integration is difficult • poor resolution of isomeric groups • CD-Screen column, reversed phase • good solubility in the eluent • β-CD contamination is the first peak, high accuracy analysis is possible • detailed „fingerprint” chromatogram . β-CD β-CD

  15. USP method for analysis of Hydroxypropylbetadex TNP: 1800/m 60 min. Alternative method on CD-Screen column TNP: 32000/m 20 min.

  16. Randomly methylated β-CD Degradation products

  17. Randomly methylated α-CD α-CD

  18. -Select • Diametrically Different Retention Mechanism • Unique Selectivity • Good alternative when current C18 fails to deliver • Diametrically different selectivity to other reversed phase columns • Embedded H-donor and H-acceptor groups • High retention of aromatics due to - interactions • Excellent shape-selectivity • 100% water can be used as eluent, no collapse • High polarity reversed phase • A single column can be used in reversed and normal phase separations • Based on “B”-type, High Purity Silica, fully endcapped • Available with 3m and 5m particle size for analytical purpose, and 10m particle size for preparative applications. • For more information please visit our web pages: www.chiroquest.org